INVESTIGATION OF SUITABLE PROTOCOL FOR IN-VITRO PROPAGATION OF Kendrickia walkeri
Asian Journal of Plant and Soil Sciences, Volume 7, Issue 1,
Kendrickia walkeri, is an epiphytic climbing shrub that belongs to the Melastomataceae family and it is vulnerable and included to the National Red List 2012 in Sri Lanka. Hence present study was aimed to find out suitable potting media for vegetative propagation and to develop suitable protocol for in-vitro propagation of Kendrickia walkeri. The pot experiment was laid out in a Completely Randomized Design (CRD) with three treatments randomized in seven replicates. Treatments were three different potting media i.e. coir dust only, coir dust: sand and coir dust: leaf mold: sand. Semi-wood stem cuttings were planted and each pot were kept in single propagators for twenty one days. After that, the data were obtained on stem height, number of new leaves, number of shoots and number of roots with two weeks intervals. Data were tabulated and analyzed using Analysis of Variance (ANOVA) procedure of Statistical Analyzing System (SAS). Duncan’s New Multiple Range Test (DNMRT) was performed to compare the differences among treatment means at p=0.05. Among different treatments the highest stem height, number of leaves/shoots as well as roots per plant was recorded in coir dust, leaf mold and sand medium. The laboratory experiments was laid out in a Completely Randomized Design (CRD) with six treatments randomized in four replicates. Treatments were different protocols i.e. 1% HgCl2 (3 minutes), 1% HgCl2 (4 minutes), 2.5 % Clorox + 1% HgCl2 (4 minutes), 5 % Clorox + 1% HgCl2 (4 minutes), 7.5 % Clorox + 1% HgCl2 (4 minutes) and 2.5 % Clorox + 1% HgCl2 (5 minutes). Nodes were treated according to the protocols and inoculated in Murashige and Skoog (MS) medium supplemented with 1.5 mg/ L benzyl amino purine (BAP). The data of number of survived explants were taken with three days intervals. The highest number of survived explants were recorded under, 2.5 % Clorox + 1% HgCl2 (5 minutes) protocol. Hence, coir dust, leaf mold and sand medium can be considered as a successful potting media for vegetative propagation of K. walkeri by nodal stem cuttings and K. walkeri exposed to 70 % ethanol for 15 seconds, 2.5% Clorox for 5 minutes and 1% HgCl2 for 5 minutes duration, can be recommended for direct plantlet regeneration from nodal explant of Kendrickia walkeri and further studies for micro propagation technique.
- Kendrickia walkeri
- vegetative propagation
- in-vitro protocol
- red list
How to Cite
Ekanayake E, Wijesundara D, Perera G. Floristic richness and the conservation value of tropical humid cloud forests of Dothalugala man and biosphere Reserve, Sri Lanka. Ceylon J Sci (Biol Sci). 2014;42(2):55.
Cooray PG. The knuckles massif – A. Forest Department, Sri Lanka: Portfolio; 1998.
Legg C. A Geographic information system for planning and managing the Conservation of tropical forest in the Knuckles Range. The Sri Lanka Forester Spec Issue. 1995:25-36.
Cruz-Cruz C, González-Arnao M, Engelmann F. Biotechnology and conservation of plant biodiversity. Resources. 2013;2(2):73-95.
Chaohua C, Gonggu Z, Lining Z, Chunsheng G, Qing T, Jianhua C et al. A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.). Ind Crops Prod. 2016;83:61-5.
Wijewickrama MMS, Gunasekera HKLK, Senaratna MMDJ, Krishnaraja SA. Effect of Cytokynin and subsequent de-topping on axillary shoot formation of Dracaena reflexa ’song of India’. Proceedings of the National Symposium on Floriculture Research. 2017; 46-51.
Meneripitiya MSK, Jayatilleke MP. Domestication of Osbeckia octandra. FESYMPO. Proceedings of the International Forestry and Environment Symposium. 2014;18.
Bandusena KWHC, Gunasekera HKLK, Krishnaraja SA. Evaluation of suitable rooting media for Bougainvillea (Bougainvillea spectabilis) stem cuttings and frequency of flowering. Proceedings of the National Symposium on Floriculture Research. 2015; 17-22.
Peiris SE, De Silva EDUD, Edussuriya M, Attanayake AMURK, Peiris BCN. CSUP technique: A novel and low cost sterilization method using sodium hypochlorite to replace the use of expensive equipment in micropropagation. J Natl Sci Found Sri Lanka. 2012;40(1):49-54.
Madurangani HGAMP, Gunasekera HKLK, Wickramasinghe MC. Investigation of best potting media to enhance flowering performance of Petunia hybrida. J Agron Agric Sci. 2020;3:2:1-6.
Nishanthan S, Gunasekera HKLK, Senavirathna KACN. Investigation of suitable growing media to enhance growth and recovery of in-vitro grown Dendrobium spp. Book of abstracts of the National Symposium on Floriculture Research (NASFLOR-2018). 2018;8.
Abstract View: 0 times
PDF Download: 0 times