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The physiological function of pepsin inhibitors could be the prevention of unwanted proteolysis and consequently control of protein turnover and metabolism. Where pepsin enzyme inhibitors reduce dietary proteins digestion and absorption. So, the aim of this research work was to inactivate pepsin presented in various crops. Pepsin inhibitors were extracted from red kidney beans seeds. The substrate was BAPA (Nα-benzoyl-L-arginine 4-nitroanilideHydrochloride) a chromogenic substrate. Phytochemical screening of red kidney beans seeds extract confirmed the presence of some phytochemical constituents e.g., saponins, glycosides, ascorbic acid, reducing compounds, carotenoids, carbohydrates, proteins, alkaloids and tannins. On the other hand, the protein concentration in the crude extract of inhibitor extracted from red kidney beans seeds was 14.05 mg/ml (17.90 mg/g, DW).
Also, the obtained results illustrated that the crude extract of red kidney beans showed inhibitory activity against pepsin enzyme. Pepsin inhibitor activity was 151.25 TUI/mg dray sample. Generally, it can be concluded that the pepsin inhibitor extracted from red kidney bean has the potential for usage as a protease inhibitor.
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