EVALUATION OF ANTI QUOROM SENSING ACTIVITIES USING Emblica officinalis ETHANOLIC LEAF EXTRACTS AGAINST MARINE MICROBES
Journal of Biochemistry International, Volume 9, Issue 4,
Page 59-66
DOI:
10.56557/jobi/2022/v9i47666
Abstract
Biofilm formation in pathogenic bacteria displays a major role in various human infectious diseases posing a major health problem in several countries. Biofilms contain colony of bacteria which are 1,000 folds more resistant to antibiotics and also they develop more insensitivity to innate and acquired immune responses. Quorum sensing (QS) is characterized well due to the interaction of bacterial cells, which is a dependent mechanism and acts its stimulus response related to population density. In the present study we tested the antiquorum sensing activity with leaf extracts of commonly used dietary supplement Emblica Officinalis (Amla) against marine microbes. In our method, we cultured Klebsilla, Proteus, E.coli, Shigella, Salmonella typhi from marine water source for quorum sensing activities and inhibitory effects through antimicrobial activities. Marine bacteria were isolated, identified based on physical and biochemical characterization. In our results we investigated the inhibitory effects of amla against pathogenic bacteria using swarming method. Further highest inhibitory bacterial isolates were examined under scanning electron microscopy (SEM). We conclude that amla exhibits potential inhibitory effects in biofilms formation from samples obtained from marine waters and further studies are needed in QS gene expression in more number of marine microbial samples.
Keywords:
- Biofilm
- Emblica officinalis
- ethanol extracts
- quorum sensing
- SEM analysis
How to Cite
NANDI, B., VIJAYALAKSHMI, K., CHRISTOPHY, A. V., HARSHAVARDHINI, G., & BABU, P. B. R. (2022). EVALUATION OF ANTI QUOROM SENSING ACTIVITIES USING Emblica officinalis ETHANOLIC LEAF EXTRACTS AGAINST MARINE MICROBES. Journal of Biochemistry International, 9(4), 59–66. https://doi.org/10.56557/jobi/2022/v9i47666
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Clay Fuqua and E. Peter Greenberg. Self Perception In Bacteria: Quorom Sensing With Acylated Homoserine Lactones. Current Opinion in Microbiology. 1998; 1(2):183-189.
Abubakar Babando Aliyu, Neil Anthony Koorbanally, Brenda Moodley, Pravesh Singh and Hafizah Yousuf Chenia. Quorom Sensing Inhibitory Potential And Molecular Docking Studies. Phytochemistry. 2016;126:23-33
Teasdale ME, Liu J, Wallace J, Akhlaghi J and Rowley DC. Secondary metabolites produced by the marine bacterium Halo bacillus salinus that inhibit quorum sensing-controlled phenotypes in Gram-negative bacteria. Applied Environmental Microbiology. 2009;75:567–572.
De Kievit TR, Gillis R and Marx S. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Applied Environmental Microbiology. 2001;67:18651873.
Musthafa KS, Ravi AV and Annapoorani A. Evaluation of antiquorum- sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy. 2010;56 (4):333-9.
Choo JH, Rukayadi Y and Hwang JK. Inhibition of bacterial quorum sensing by vanilla extract. Letters in Applied Microbiology. 2006;42:637-41.
Germano MP, De Pasquale, R, D’Angelo V. Evaluation of extracts and isolated fraction from Capparisspinosa L. buds as antioxidant source. Journal of Agricultural and Food Chemistry. 2002;50:1168-1171.
Packiavathy I, Agilandeswari P and Musthafa K. Antibiofilm and quorum sensing inhibitory potential of cuminumcyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food Research International. 2012;45(1):85–92.
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Annapoorani, Angusamy et al. “Inhibition of Quorum Sensing Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens PS1 by Marine Sponges.” Indian journal of microbiology. 2012;52(2):160-6.
Issac Abraham SV, Palani A, Ramaswamy BR, Shunmugiah KP, Arumugam VR. Antiquorum sensing and antibiofilm potential of Capparis spinosa. Arch Med Res. 2011;42(8):658-68.
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Rogers SA, Huigens RW 3rd, Cavanagh J, Melander C. Synergistic effects between conventional antibiotics and 2-aminoimidazole-derived antibiofilm agents. Antimicrob Agents Chemother. 2010;54(5):2112-8.
Thenmozhi R, Nithyanand P, Rathna J and Pandian SK. Antibiofilm activity of coral-associated bacteria against different clinical M serotypes of Streptococcus pyogenes, FEMS Immunology and Medical Microbiology. 2009;57:284–294.
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Halkare SuryanarayanaVasavi, Anantha Padmanabha Bhagwa Tharun, Punchapady-Devasyarekha. Anti-quorom sensing activity of psidium guajava l. flavonoids against chromobacterium violaceum and pseudomonas aeruginosa PA01. Microbiology and Immunology. 2014;58(5):286-293.
Beatriz Jaramillo-Colorado, Jesusolivero-Verbel, Elena E. Stashenko, Irene Wangner. Anti-quorom sensing activity of essential oils from Colombian plants. Natural product research. 2012;26(12):1075-1086.
Issac Abraham Sybiya Vasantha Packiavathy, Palani Agilandeshwari, Khaddar Syedmushtafa, Shunmugi Ahkarutha Pandian, Arumugam Veera Ravi. Antibiofilm and quorum sensing inhibitory potential of cuminum cyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food research international. 2012. Volume 45, issue 1, pages 85-92.
Fratianni F, Coppola R, Nazzaro F. Phenolic composition and antimicrobial and antiquorum sensing activity of an ethanolic extract of peels from the apple cultivar Annurca. J Med Food. 2011;14(9):957-63
Kshipranaik andMeenal Kowshik. Anti-quorom sensing activity of agcl-tio2 nanoparticles with potential use as active food packaging material. Journal of Applied Microbiology. 2017;117(4):972-983.
Brackman G, Cos P, Maes L, Nelishj, Coenye T. Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. Antimicrob agents chemother. 2011. 55 (6):2655-61.
Deep A, Chaudhary U, Gupta V. Quorum sensing and bacterial pathogenicity: From molecules to disease. J Lab Physicians. 2011;3:4–11.
Kabir AH, Roy AG, Alam MF, Islam R. Detection of quorum sensing signals in gram-negative bacteria by using reporter strain CV026. Notulae Sci Biol. 2010;2:72–5.
Krishnan T, Yin WF, Chan KG. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa PAO1 by Ayurveda spice clove (Syzygium aromaticum) bud extract. Sensors (Basel). 2012;12:4016–30.
Clay Fuqua and E. Peter Greenberg. Self Perception In Bacteria: Quorom Sensing With Acylated Homoserine Lactones. Current Opinion in Microbiology. 1998; 1(2):183-189.
Abubakar Babando Aliyu, Neil Anthony Koorbanally, Brenda Moodley, Pravesh Singh and Hafizah Yousuf Chenia. Quorom Sensing Inhibitory Potential And Molecular Docking Studies. Phytochemistry. 2016;126:23-33
Teasdale ME, Liu J, Wallace J, Akhlaghi J and Rowley DC. Secondary metabolites produced by the marine bacterium Halo bacillus salinus that inhibit quorum sensing-controlled phenotypes in Gram-negative bacteria. Applied Environmental Microbiology. 2009;75:567–572.
De Kievit TR, Gillis R and Marx S. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Applied Environmental Microbiology. 2001;67:18651873.
Musthafa KS, Ravi AV and Annapoorani A. Evaluation of antiquorum- sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy. 2010;56 (4):333-9.
Choo JH, Rukayadi Y and Hwang JK. Inhibition of bacterial quorum sensing by vanilla extract. Letters in Applied Microbiology. 2006;42:637-41.
Germano MP, De Pasquale, R, D’Angelo V. Evaluation of extracts and isolated fraction from Capparisspinosa L. buds as antioxidant source. Journal of Agricultural and Food Chemistry. 2002;50:1168-1171.
Packiavathy I, Agilandeswari P and Musthafa K. Antibiofilm and quorum sensing inhibitory potential of cuminumcyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food Research International. 2012;45(1):85–92.
Deziel E, Comeau Y and Villemur R. Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities. Journal of Bacteriology. 2001.183:1195-1204.
Bjarnsholt T, Jensen PØ, Rasmussen TB, Christophersen L, Calum H, Hentzer M, Hougen HP, Rygaard J, Moser C, Eberl L, Høiby N, Givskov M. Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology (Reading). 2005;151(Pt 12):3873-3880.
Annapoorani, Angusamy et al. “Inhibition of Quorum Sensing Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens PS1 by Marine Sponges.” Indian journal of microbiology. 2012;52(2):160-6.
Issac Abraham SV, Palani A, Ramaswamy BR, Shunmugiah KP, Arumugam VR. Antiquorum sensing and antibiofilm potential of Capparis spinosa. Arch Med Res. 2011;42(8):658-68.
Ali-Shtayeh MS and Abu-Ghdeib SI. Antifungal activity of plant extracts against dermatophytes, Mycoses Journal. 1999;42:665-672.
Adonizio A, Kong KF, Mathee K. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Chemother. 2008 Jan; 52(1):198- 203.
Rogers SA, Huigens RW 3rd, Cavanagh J, Melander C. Synergistic effects between conventional antibiotics and 2-aminoimidazole-derived antibiofilm agents. Antimicrob Agents Chemother. 2010;54(5):2112-8.
Thenmozhi R, Nithyanand P, Rathna J and Pandian SK. Antibiofilm activity of coral-associated bacteria against different clinical M serotypes of Streptococcus pyogenes, FEMS Immunology and Medical Microbiology. 2009;57:284–294.
Allison L. Adonizio, Kelsey Downum, Bradley C Bennet, Kalai Mathee. Anti Qourom Sensing Activity Of Medicinal Plants In Southern Florida. Journal Of Ethnopharmacology. 2006. 105 (3), 427-435.
Maria v. alvarez, Maria R. Moreira, Alejandra Ponce. Anti qourom sensing and antimicrobial activity of natural agents with potential use in food. Journal of Food Safety. 2012;32(3):379-387.
Maryam Zahin, Sameenahasan, Farrukaqil, Sajjadahmad Khan, Fohad mabood hussain, Iqbalahmad. Screening of certain medicinal plants from India for their anti-quorom sensing activity. Indian Journal of Experimental Biology. 2010;48:1219-1224.
Md. Akilhossain, Ji-Yong Park, Jin-Yoonkim, Joo-Won Suh, Seung-Chun Park. Synergistic effect and antiquorom sensing activity of nymphaea tetragona (water lily) extract. Biomed Research International. 2014; article ID 562173:10.
Weihuachu, Shuxinzhou, Yanjiang, Weizhu, Xiyizhuang, Jiangyanfu. Effect of traditional chinese herbal medicine with antiquorom sensing activity on pseudomonas aeruginosa. Evidence-based Complementary and Alternative Medicine. 2013; article ID 648257:7.
Halkare SuryanarayanaVasavi, Anantha Padmanabha Bhagwa Tharun, Punchapady-Devasyarekha. Anti-quorom sensing activity of psidium guajava l. flavonoids against chromobacterium violaceum and pseudomonas aeruginosa PA01. Microbiology and Immunology. 2014;58(5):286-293.
Beatriz Jaramillo-Colorado, Jesusolivero-Verbel, Elena E. Stashenko, Irene Wangner. Anti-quorom sensing activity of essential oils from Colombian plants. Natural product research. 2012;26(12):1075-1086.
Issac Abraham Sybiya Vasantha Packiavathy, Palani Agilandeshwari, Khaddar Syedmushtafa, Shunmugi Ahkarutha Pandian, Arumugam Veera Ravi. Antibiofilm and quorum sensing inhibitory potential of cuminum cyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food research international. 2012. Volume 45, issue 1, pages 85-92.
Fratianni F, Coppola R, Nazzaro F. Phenolic composition and antimicrobial and antiquorum sensing activity of an ethanolic extract of peels from the apple cultivar Annurca. J Med Food. 2011;14(9):957-63
Kshipranaik andMeenal Kowshik. Anti-quorom sensing activity of agcl-tio2 nanoparticles with potential use as active food packaging material. Journal of Applied Microbiology. 2017;117(4):972-983.
Brackman G, Cos P, Maes L, Nelishj, Coenye T. Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. Antimicrob agents chemother. 2011. 55 (6):2655-61.
Deep A, Chaudhary U, Gupta V. Quorum sensing and bacterial pathogenicity: From molecules to disease. J Lab Physicians. 2011;3:4–11.
Kabir AH, Roy AG, Alam MF, Islam R. Detection of quorum sensing signals in gram-negative bacteria by using reporter strain CV026. Notulae Sci Biol. 2010;2:72–5.
Krishnan T, Yin WF, Chan KG. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa PAO1 by Ayurveda spice clove (Syzygium aromaticum) bud extract. Sensors (Basel). 2012;12:4016–30.
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