CORRELATION OF CAPN10 POLYMORPHISM (rs3792267) WITH HYPERTENSION IN DIABETIC PATIENTS FROM IRAQ
Journal of Disease and Global Health,
Background: Calpain-10 is calcium-activated cysteine intracellular proteases, encoded by CAPN10. Recently, the CAPN10 Polymorphism (rs3792267) have been shown to be associated with type 2 diabetes mellitus (T2DM). The purpose of our study was to examine the association of this polymorphism (rs3792267) with coexisting hypertension in Iraqi individuals, affected by T2DM.
Methods: A case-control study was conducted, examining the association of the SNP, rs3792267 with T2DM, with and without hypertension. Of the total two hundred cases, 100 T2DM subjects with hypertension, and 100 T2DM individuals without hypertension, used as the patients and controls, respectively. They were selected randomly based on the WHO criteria. DNA was extracted from the blood, and genotyped by PCR-RFLP, using the restriction enzyme NdeI. Then, the PCR product was resolved on agarose gel electrophoresis.
Results: Analysis of the genotype and allele frequencies of CAPN10 polymorphism (rs3792267) revealed that the heterozygous genotype (AG) was significantly different (p=0.0001), while homozygous genotype (AA) shows no significant difference between the patient and control groups. The frequency of the A allele of the rs3792267 polymorphism was significantly different between the two groups (p=0.0001), 36.5% without hypertension and 15.5% with hypertension, (OR=0.1662, CI% 0.0901-0.3064), indicating that the A allele is associated with T2DM. On the other hand, no significant statistical difference was observed in the mean values of cholesterol and triglycerides of T2DM, with and without hypertension.
Conclusion: Although the polymorphism (rs3792267) in the CAPN10 appears to have a hereditary relationship with the pathogenesis of type 2 diabetic patients, but it was not linked with the increased risk of hypertension in T2DM.
- Type 2 diabetes mellitus
How to Cite
Yan S, Li C, Tian H, Li J, Pei Y, Liu Y, et al. Association of calpain-10 rs2975760 polymorphism with type 2 diabetes mellitus: A meta-analysis. Int J Clin Exp Med. 2014;7(10): 3800–7.
Maryam Rezazadeh, Nasim Sohrabifar, Hossein Jafari, Mojtaba Mohaddes, Ardabili JG. A study on Association of polymorphisms in Calpain10 and TCF7L2 genes with type 2 diabetes mellitus. Genetika. 2015;47(1):161–70.
Ezzidi I, Turki A, Messaoudi S, Chaieb M, Kacem M, Al-khateeb GM, et al. Common polymorphisms of calpain-10 and the risk of type 2 diabetes in a Tunisian Arab population: A case-control study. BMC Med Genet. 2010; 11(75):8–10.
Laakso M. JP. US. MV, ER. JK. IVSK. MCYN. NJCG. B. Common polymorphisms of calpain-10 are associated with abdominal obesity in subjects at high risk of type 2 diabetes. Diabetologia. 2006;49:1560–6.
Carlsson E, Fredriksson J, Groop L, Ridderstråle M. Variation in the Calpain-10 gene is associated with elevated triglyceride levels and reduced adipose tissue messenger ribonucleic acid expression in obese Swedish subjects. J Clin Endocrinol Metab. 2004;89(7): 3601–5.
Ali I, Movva S, Ahmad N, Chava S. Investigation of Calpain 10 ( rs2975760 ) gene polymorphism in Asian Indians with gestational diabetes mellitus. Turkish J Med Sci [Internet]. Elsevier B.V. 2017;2:299–306.
Tsai H, Sun G, Weeks DE, Kaushal R, Wolujewicz M, Mcgarvey ST, et al. Type 2 diabetes and three Calpain-10 gene polymorphisms in Samoans: No evidence of association. Am J Hum Genet. 2001;69:1236–44.
Organización Mundial de la Salud. Guidelines for the collection of clinical specimens during field investigation of outbreaks. Encycl Disabil [Internet]. 2006;1–5.
Rasmussen SK, Urhammer SA, Berglund L, Jensen JN, Hansen L, Echwald SM, et al. Variants within the Calpain-10 gene on chromosome secretion among Scandinavian Caucasians. Diabetes. 2002;51(12):3561–7.
Senemar S, Edraki MR, Toosi S. Association between type 2 diabetes mellitus, biochemical factors and UCSNP-43 polymorphisms of CALPIN-10 gene in patients with athero-sclerosis of coronary artery disease in Southern Iran population. J Cardiovasc Thorac Res [Internet]. 2016;8(1):13–9.
Naciye Selcen Bayramci, Leyla Açik, Çağdaş Kalkan Iy. Investigation of glucocorticoid receptor and calpain-10 gene polymorphisms in Turkish patients with type 2 diabetes mellitus. Turkish J Med Sci. 2017;47:1568–75.
Maleki F, Haghani K, Shokouhi S, Mahmoodi K, Sayehmiri K, Mahdieh N, et al. A case-control study on the association of common variants of CAPN10 gene and the risk of type 2 diabetes in an Iranian population. Clin Lab. 2014;663–70.
Parra EJ, Hoggart CJ, Bonilla C, Dios S, Norris JM, Marshall JA, Hamman RF, Ref PM, McKeigue MDS. Relation of type 2 diabetes to individual admixture and candidate gene polymorphisms in the Hispanic American population of San Luis Valley, Colorado. J Med Genet. 2004;10:1–9.
Abstract View: 1036 times
PDF Download: 4 times