COLLECTION, ISOLATION, PURIFICATION AND IDENTIFICATION OF MICROALGAE FROM PADDY SOIL AT KADAWA IRRIGATION FARMLAND, KANO STATE
Journal of Global Ecology and Environment,
Page 1-11
Abstract
The success of any microalgal research involves effective methods for sampling environments, isolation, purification and the maintenance of algal strains. The isolation of soil microalgae poses a great challenge since the soil is a home for thousands of different species, including algal species, bacteria, viruses and fungi. As such cultures are susceptible to contamination, which may interfere with algal structure, biomass yield and the erroneous identification of species for molecular genetic analysis. The most common methods for isolation and purification of microalgae are the single cell isolation, serial dilution and a streak plate. This procedure is tedious, time consuming, demands precision and expertise and may not yield results. Thus in the present study, an attempt was made to modify these methods for the isolation of soil microalgae. With the modification, three microalgal sp. were isolated, purified and identified (viz Chlorella vulgaris, Microcystis aeruginosa and Scenedesmus obliquus) in a simplified manner.
Keywords:
- Microalgae
- isolation
- purification
- modified method
How to Cite
AFIYA, H., & UMAR, K. M. (2022). COLLECTION, ISOLATION, PURIFICATION AND IDENTIFICATION OF MICROALGAE FROM PADDY SOIL AT KADAWA IRRIGATION FARMLAND, KANO STATE. Journal of Global Ecology and Environment, 16(3), 1-11. Retrieved from https://ikppress.org/index.php/JOGEE/article/view/7707
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Morais M, Radmann E, Costa J. Biofixation of CO2 From Synthetic Combustion Gas Using Cultivated Microalgae in Three-Stage Serial Tubular Photobioreactors. Journal of Bioscience. 2011;66:313-8.
Xiong J, Govindwar S, Kurade M, Paeng K, Roh H, Khan M, Jeon B. Toxicity of Sulfamethazine and Sulfamethoxazole and Their Removal by a Green Microalga, Scenedesmus Obliquus. Chemosphere. 2019;218:551-558.
Du K, Wen X, Wang Z, Liang F, Luo L, Peng X, Xu Y, Geng Y, Li Y. Integrated Lipid Production, CO 2 Fixation, and Removal of SO 2 and NO From Simulated Flue Gas by Oleaginous Chlorella Pyrenoidosa. Environmental Science and Pollution Research International. 2019;26:16195-16209.
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Rzymski P, Niedzielski P, Karczewski J, Poniedziałek B. Biosorption of toxic metals using freely suspended Microcystis aeruginosa biomass. Central European Journal of Chemistry. 2014;12.
Roberts C. Boylen, Nierzwicki-Bauer S. Effects of Lead Accumulation on the Azolla caroliniana-Anabaena Association," Ecotoxicology and Environmental safety. 2014;102:100-4.
Fawzy M, Issa A. Bioremoval of Heavy Metals and Nutrients From Sewage Plant by Anabaena Oryzae and Cyanosarcina Fontana. International Journal of Phytoremediation. 2016;18(4):321-8.
X. Shen, H. Zhang, X. He, H. Shi, C. Stephan, H. Jiang, C. Wan and T. Eichholz, "Evaluating the treatment effectiveness of copper-based algaecides on toxic algae Microcystis aeruginosa using single cell-inductively coupled plasma-mass spectrometry," Analytical and Bioanalytical Chemistry, vol. 411, p. 5531–5543, 2019.
C. Gao, L. Gao, P. Duan, H. Wu and M. Li, "Evaluating combined toxicity of binary heavy metals to the cyanobacterium Microcystis: A theoretical non-linear combined toxicity assessment method," Ecotoxicology and Environmental Safety, vol. 187, 2020.
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Z. Yang, L. Y, X. Fu, J. Zaporski, S. Peters, M. Jamison, L. Y, S. Wullschleger, D. Graham and B. Gu, "Temperature Sensitivity of Mineral-Enzyme Interactions on the Hydrolysis of Cellobiose and Indican by β-glucosidase," The Science of the total Enviroment, vol. 10, p. 686, 2019.
P. Zuccarini, D. Asensio, R. Ogaya, J. Sardans and J. Peñuela, "Effects of Seasonal and Decadal Warming on Soil Enzymatic Activity in a P-deficient Mediterranean Shrubland," Global Change Biology, vol. 26(6), pp. 3698-371, 2020.
Carmo D, Silva C, Lima J, Pinheiro G. Electrical Conductivity and Chemical Composition of Soil Solution: Comparison of Solution Samplers in Tropical Soils. Revista Brasileira de Ciência do Solo. 2016;40.
Gogoi P, Sinha A, Sarkar S, Chanu T, Yadav AK, Koushlesh S, Borah S, Das S, Das B. Seasonal infuence of physicochemical parameters on phytoplankton diversity and assemblage pattern in Kailash Khal, a tropical wetland, Sundarbans, India. Applied Water Science. 2019;9:156.
Sigamani S, Ramamurthy D, Natarajan H. A review on potential biotechnological applications of microalgae. Journal of Applied Pharmaceutical Science. 2016;6(8):179-184.
Blinova L, Bartosoba A, Gerulova K. Cultivation of Microalgae Equation (Chlorella vulgaris) for Biodeiel production," Research papers; 2015.
Mata T, Martins A, Caetano N. Microalgae for biodiesel production and other Applications. Renewable Sustainable Energy. 2010;14:217-232.
Yan N, Fan C, Chen Y, Hu Z. The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals," International Journal of Molecular Sciences. 2016;17:962.
Mobin S, Alam F. Some promising microalgal species for commercial applications: A review. In 1st International Conference on Energy and Power; 2017.
Sathasivam R, Radhakrishnan R, Hashem A, Abdallah E. Microalgae metabolites: A rich source for food and medicine. Saudi Journal of Biological Sciences; 2018.
Temraleeva SA, Dronova S Moskalenko, Didovich SV. Modern methods for isolation, purification and cultivation of soil Cyano bacteria. Microbiology. 2016;85(4):369-380.
Park J, Graggs R, Shilton A. Wastewater treatment high rate algal ponds for biofuel production. Bioresourse Technology. 2011;102:35-42.
Parvin M, Zannat M, Habib M. Two important techniques for isolation of microalgae. Asian Fisheries Science. 2007;20:117-124.
Saadatnia H, Riahi H. Cyanobacteria from paddy fields in Iran as a biofertilizer in rice plants. Plant Soil Environment. 2009;55:207–212.
Singh P, Gupta S, Guldhe A, Rawat I, Bux F.. "Microalgae Isolation and Basic Culturing Techniques," in Handbook of Marine Algae, Elsevier; 2015.
Ye S, Gao L, Zhao J, An M, Wu H, Li M. Simultaneous wastewater treatment and lipid production byScenedesmussp.HXY2," Bioresource Technology. 2020;302.
Manual for Describing Soils in the Field; 2007.
Chowdhury MA. Determination of water content of soil sample by different method. In North East Cogress Students Geo Congress on Advaances in Geotechnical Engineering; 2014.
Ma M, Zang Z, Xie Z, Chen Q, Xu W, Zhao C, Shen G. Soil respiration of four forests along elevation gradient in northern subtropical China. Ecology & Evolution; 2019.
Masaki O, Takenaka M, Sato Y, Smith R, Inomata H. Effects of light intensity and temperature on photoautotrophic growthof a green microalga, Chlorococcum littorale. Biotechnology Reports. 2015;7:24-29.
West J. Long-Term Macroalgal Culture Maintenance, R. Anderson, Ed., California: Elsevier Academic Press; 2005.
Janse Van vuuren S, Taylor J, Gerber A, Van Ginkel C. Easy identification of the most common fresh water Algae: A guide for the identification of microscopic algae in South African Freshwaters; 2006.
Prescott G. The fresh water Algae, Michigam: WM. G Brown Company; 1954.
Cortés S. Sánchez-Fortún, M. García and C. Bartolomé, "Effects of pH on the growth rate exhibited of the wild-type andCd-resistant Dictyosphaerium chlorelloides strains. Limnetica. 2018;37(2):229-238.
Darvehei P, Bahri P, Moheimani N. Modeling the effect of temperature on microalgal growth under outdoor conditions. Computer Aided Chemical Engineering. 2018;43:55-60.
Nandigam J, Rangaiah S, Geddada M. A study on Seasonal changes of microalgae in reponse to the Physicochemical Parameters of Satyavaram Pond, Srikakulam Dist, India. Indian Journal of Geo Marine Sciences. 2016;45(12):1660-1668.
Chen R, Ju M, Chu C, Jing W, Wang Y. Identification and Quantification of Physicochemical Parameters Influencing Chlorophyll-a Concentrations through Combined Principal Component Analysis and Factor Analysis: A Case Study of the Yuqiao," Sustainability. 2018;10:936.
Myint M, Hussein W, Ghassemi W. Microalgal process for treatment of high conductivity concentrates from inland desalination. Desalination and water Treatment. 2016;57.
Figler V, B-Béres D. Dobronoki, Márton K, Nagy SA, I. Bácsi. Salt Tolerance and Desalination Abilities of Nine Common Green Microalgae Isolates. Water. 2019;11: 2527.
Taziki M, Ahmadzadeh H, Murry-Ewers M, S. Lyon, "Nitrate and Nitrite Removal from Wastewater using Algae," Current Biotechnology. 2015;4.
Singh D, Nedbal L, Ebenhöh O. Modelling phosphorus uptake in microalgae. Biochemical Society Transactions. 2018;46:483–490.
Sayadi M, Ahmadpour N, Fallahi CM, Rezaei M. Removal of nitrate and phosphate from aqueous solutions by microalgae:An experimental study. Global Journal of Environmental Science Management. 2016;2 (3):357-364.
Gutwiński P, Cema G. Removal of nitrogen and phosphorus from reject water using chlorella vulgaris algae after partial nitrification/anammox process. Water Environment Research. 2016;88(1):63 -69.
Sabeti M, Hejazi MA, Karimi A. Enhanced removal of nitrate and phosphate from wastewater by Chlorella vulgaris: Multi-objective optimization and CFD simulation. Chinese Journal of Chemical Engineering. 2019;27:639-648.
Morais M, Radmann E, Costa J. Biofixation of CO2 From Synthetic Combustion Gas Using Cultivated Microalgae in Three-Stage Serial Tubular Photobioreactors. Journal of Bioscience. 2011;66:313-8.
Xiong J, Govindwar S, Kurade M, Paeng K, Roh H, Khan M, Jeon B. Toxicity of Sulfamethazine and Sulfamethoxazole and Their Removal by a Green Microalga, Scenedesmus Obliquus. Chemosphere. 2019;218:551-558.
Du K, Wen X, Wang Z, Liang F, Luo L, Peng X, Xu Y, Geng Y, Li Y. Integrated Lipid Production, CO 2 Fixation, and Removal of SO 2 and NO From Simulated Flue Gas by Oleaginous Chlorella Pyrenoidosa. Environmental Science and Pollution Research International. 2019;26:16195-16209.
Shahid S Malik H, Zhu J, Xu M, Nawaz S, Nawaz M Alam M. Mehmood. Cultivating Microalgae in Wastewater for Biomass Production, Pollutant Removal, and Atmospheric Carbon Mitigation; A Review," The Science of the total Environment. 2020;704:13530.
Salama E, Roh H, Dev S, Khan M, Abou-Shanab R, Chang S, Jeon B. Algae as a Green Technology for Heavy Metals Removal From Various Wastewater. World Journal of Microbiology and Biotechnology. 2019;35:75.
Jin Z, DaYong Z, Yong Ban J, QingLong W. Comparison of heavy metal accumulation by a bloom-forming cyanobacterium, Microcystis aeruginosa. Chinese Science Bulletin. 2012;57:3790-3797.
Rzymski P, Niedzielski P, Karczewski J, Poniedziałek B. Biosorption of toxic metals using freely suspended Microcystis aeruginosa biomass. Central European Journal of Chemistry. 2014;12.
Roberts C. Boylen, Nierzwicki-Bauer S. Effects of Lead Accumulation on the Azolla caroliniana-Anabaena Association," Ecotoxicology and Environmental safety. 2014;102:100-4.
Fawzy M, Issa A. Bioremoval of Heavy Metals and Nutrients From Sewage Plant by Anabaena Oryzae and Cyanosarcina Fontana. International Journal of Phytoremediation. 2016;18(4):321-8.
X. Shen, H. Zhang, X. He, H. Shi, C. Stephan, H. Jiang, C. Wan and T. Eichholz, "Evaluating the treatment effectiveness of copper-based algaecides on toxic algae Microcystis aeruginosa using single cell-inductively coupled plasma-mass spectrometry," Analytical and Bioanalytical Chemistry, vol. 411, p. 5531–5543, 2019.
C. Gao, L. Gao, P. Duan, H. Wu and M. Li, "Evaluating combined toxicity of binary heavy metals to the cyanobacterium Microcystis: A theoretical non-linear combined toxicity assessment method," Ecotoxicology and Environmental Safety, vol. 187, 2020.
C. Tang and D. Chen, "Interaction between Soil Moisture and Air Temperature in the Mississippi River Basin," Environmental Protection Agency, vol. 9(10), p. 1119–1131, 2017.
Z. Yang, L. Y, X. Fu, J. Zaporski, S. Peters, M. Jamison, L. Y, S. Wullschleger, D. Graham and B. Gu, "Temperature Sensitivity of Mineral-Enzyme Interactions on the Hydrolysis of Cellobiose and Indican by β-glucosidase," The Science of the total Enviroment, vol. 10, p. 686, 2019.
P. Zuccarini, D. Asensio, R. Ogaya, J. Sardans and J. Peñuela, "Effects of Seasonal and Decadal Warming on Soil Enzymatic Activity in a P-deficient Mediterranean Shrubland," Global Change Biology, vol. 26(6), pp. 3698-371, 2020.
Carmo D, Silva C, Lima J, Pinheiro G. Electrical Conductivity and Chemical Composition of Soil Solution: Comparison of Solution Samplers in Tropical Soils. Revista Brasileira de Ciência do Solo. 2016;40.
Gogoi P, Sinha A, Sarkar S, Chanu T, Yadav AK, Koushlesh S, Borah S, Das S, Das B. Seasonal infuence of physicochemical parameters on phytoplankton diversity and assemblage pattern in Kailash Khal, a tropical wetland, Sundarbans, India. Applied Water Science. 2019;9:156.
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