In vitro MORPHOGENESIS OF FOLIAR EXPLANTS AND PLANT REGENERATION OF Actinidia deliciosa A. Chev. – A HORTICULTURAL IMPORTANT PLANT
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 21, Issue 15-16,
Page 114-123
Abstract
Actinidia deliciosa or Kiwi is an important horticultural plant. Common practice of propagation of the species is through stem cutting, but poor rooting of stem cuttings limits the production of clonal planting materials. Present study aimed at development of an in vitro propagation protocol using in vitro source leaves. About 5-8 week old leaves (intact, segments and scrubbed leaves) were cultured on MS medium fortified with BA (9 µM) and sucrose (3%), where within 5-6 days of culture morphogenic response initiated and after 4 week of culture 50.5% intact leaves responded with 5 shoot buds formation; while, 41.7 and 41.6% leaf segments and scrubbed leaf segments respectively responded and registered each 7 shoot buds formation. Shoot buds proliferated and developed multiple micro shoots on MS medium with sucrose (3%) and BA (3 µM) where as many as 6.2 micro shoots developed in 58.3% culture per cycle. Well-developed foliated and defoliated micro shoots (4-5 cm long) were rooted on nutrient medium containing IBA (9 µM) where 50 and 56.25% rooting response registered respectively accompanied by 5 and 7 numbers of roots per shoot. The rooted plantlets were hardened on 1/4th strength liquid medium containing sucrose (1%) for 7-8 week followed by transferred to potting mix where ~82% transplants survived and 70% transplants established in the field.
Keywords:
- Clonal propagation
- defoliation and rooting
- foliar explants
- Kiwi
How to Cite
DEB, C. R., & GANGMEI, P. K. (2020). In vitro MORPHOGENESIS OF FOLIAR EXPLANTS AND PLANT REGENERATION OF Actinidia deliciosa A. Chev. – A HORTICULTURAL IMPORTANT PLANT. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(15-16), 114–123. Retrieved from https://ikppress.org/index.php/PCBMB/article/view/5174
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Deb CR, Gangmei PK. Callus mediated indirect somatic embryogenesis and plant regeneration of Saurauia punduana Wallich (Actinidiaceae) from in vitro cotyledonary leaves. Curr. Biotechnol. 2017;6:69-76.
Deb CR, Gangmei PK. Shoot regeneration from nodal segments of Saurauia punduana Wallich: A threatened therapeutically important plant. Plant Cell Biotechnol. Mol. Biol. 2017;18(3&4):90-100.
Deb CR, Gangmei PK. In vitro propagation of Kiwi from nodal segments and development of a new rooting technique of micro shoots. Biotechnol. 2019;18:64-76.
Zaki M, Kaloo ZA, Sofi MS. Micropropagation of Morus nigra L. from nodal segments with axillary buds. World J. Agricult. Sci. 2011;7(4):496-503.
Deb CR, Arenmongla T. An efficient in vitro regeneration protocol for a natural dye yielding plant, Strobilanthes flaccidifolious Nees., from nodal explants. Indian J. Exp. Biol. 2012;50:810-816.
Deb MS, Jamir NS, Deb CR. In vitro culture of embryos of Cinnamomum tamala Nees.- the role of different factors. Indian J. Exp. Biol. 2014;52:1003-1010.
Deb CR, Rout GR, Mao AA, Nandi SK, Singha RKN, Vijayan D, Langhu T, Kikon ZP, Pradhan S, Tariq M, Swain D. In vitro propagation of some threatened plant species of India. Curr. Sci. 2018; 114(3):567-575.
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Akbas FA, Isikalan C, Namli S, Basaran D. Micropropagation of Kiwi fruit (Actinidia deliciosa). Intl. J. Agricult. Biol. 2007;9(3):489-493.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-497.
Omar MS, Hameed MK, Al-Rawi MS. Micropropagation of date palm (Phoenix dactylafera L.). In: Bajaj, Y. P. S. (ed.), Biotechnology in Agriculture and Foresty Vol. 18. High. Technology and Micropropagation II. Springer-Verlag, Berlin. 1992;471-492.
Caldas LS, Haridasan P, Ferreira ME. Rooting and acclimatization of micropropagated Gypsophila paniculata plants. Hortc. Braz. 1998;l(1):87-132.
Nowak B, Miczynski K, Hudy L. Sugar uptake and utilization during adventitious bud differentiation on in vitro leaf explant of Wegierka zwykla plum (Prunus domestica). Pl. Cell Tiss. Org. Cult. 2004;76(3):255-260.
Siwach P, Grover K, Gill AR. The influence of plant growth regulators explants nature and sucrose concentration on in vitro callus growth of Thevetia peruviana Schum. Asian J. Biotechnol. 2011; 3(3):280-292.
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Thangavel P, John Britto S, Senthilkumar SR. Adventitious shoot regeneration from leaf explants of the valuable medicinal herb Plectranthus barbatus Andrews. Afr. J. Biotechnol. 2011;10(43):8562‐8569.
Hamidoghli Y, Sharaf ARN, Zakizadeh H. Organogenesis from seedling derived leaf explants of primrose (Primula heterochroma Stapf.) as a scarce Iranian plant. Aus. J. Crop Sci. 2011;5(4):391-395.
Meng R, Chen THH, Finn CE, Li J. Improving in vitro plant regeneration from leaf and petiole explants of ‘Marion’ blackberry. Hortc. Sci. 2004;39(2):316-320.
Lo KH, Giles KL, Sawhney VK. Acquisition of competence for shoot regeneration in leaf discs of Saintpaulia ionantha × confusa hybrids (African violet) cultured in vitro. Pl. Cell Rep. 1997;16:416–420.
Taha HS, Hassan MM, El-Bahr MK. Micro propagation of some Egyptian date palm dry cultivars, 1- Maturation of somatic embryos. Arab. J. Biotechnol. 2007;10(2):333-340.
Muniran F, Bhore SJ, Shah FH. Micropropagation of Elaeis guineensis Jacq. ‘Dura’: comparison of three basal media for efficient regeneration. Indian J. Exp. Biol. 2008;46(1):79-82.
Placide R, Clement U, Fracoise U, Vedaste A. Comparative study of effects of table sugar, laboratory grade sucrose and mannitol on growth of banana plantlets under in vitro conditions. Rwanda J. Agri. Sci. 2012;28:76-83.
Meenakshi S, Shinde BN, Suprasanna P. Somatic embryogenesis from immature male flowers and molecular analysis of regenerated plants in banana “Lal Kela” (AAA). Fruit Ornamental Pl. Res. 2011;19(2):15-30.
Thakur R, Rao PS, Bapat VA. In vitro plant regeneration in Melia azedarach L. Pl. Cell Rep. 1998;18:127-131.
Aliou N, Mamadou SD, Dame N, Yaye Kene GD. In vitro regeneration of adult trees of Bambusa vulgaris. Afr. J. Biotechnol. 2006;5:1245-1248.
Vengadesan G, Ganapathi A, Anand RP, Anbazhagan VR. In vitro propagation of Acacia sinuata (Lour.) Merr. via cotyledonary nodes. Agro. For. Systems. 2002;55:9-15.
Nanda RM, Das P, Rout GR. In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annl. For. Sci. 2004;61:381-396.
Pospisilova J, Ticha, I, Kadlecek P, Haisel D, Plzakova S. Acclimatization of micropropagated plants to ex vitro conditions. Biol. Plant. 1999;42:481- 497.
Deb CR, Imchen T. An efficient in vitro hardening technique of tissue culture raised plants. Biotechnol. 2010;9(1):79-83.
Deb CR, Pongener A. A study on the use of low cost substrata against agar for non-symbiotic seed culture of Cymbidium iridioides D. Don. Aus. J. Crop Sci. 2013;7(5):642-649.
Deb CR, Sangtam TL, Jamir NS. Clonal macropropagation of Bumbusa nagalandiana Naithani through culm segments and branch cutting: an endemic bamboo of Nagaland, India. Intl. J. Conserv. Sci. 2016;7(1):147-154.
Deb CR, Gangmei PK. Callus mediated indirect somatic embryogenesis and plant regeneration of Saurauia punduana Wallich (Actinidiaceae) from in vitro cotyledonary leaves. Curr. Biotechnol. 2017;6:69-76.
Deb CR, Gangmei PK. Shoot regeneration from nodal segments of Saurauia punduana Wallich: A threatened therapeutically important plant. Plant Cell Biotechnol. Mol. Biol. 2017;18(3&4):90-100.
Deb CR, Gangmei PK. In vitro propagation of Kiwi from nodal segments and development of a new rooting technique of micro shoots. Biotechnol. 2019;18:64-76.
Zaki M, Kaloo ZA, Sofi MS. Micropropagation of Morus nigra L. from nodal segments with axillary buds. World J. Agricult. Sci. 2011;7(4):496-503.
Deb CR, Arenmongla T. An efficient in vitro regeneration protocol for a natural dye yielding plant, Strobilanthes flaccidifolious Nees., from nodal explants. Indian J. Exp. Biol. 2012;50:810-816.
Deb MS, Jamir NS, Deb CR. In vitro culture of embryos of Cinnamomum tamala Nees.- the role of different factors. Indian J. Exp. Biol. 2014;52:1003-1010.
Deb CR, Rout GR, Mao AA, Nandi SK, Singha RKN, Vijayan D, Langhu T, Kikon ZP, Pradhan S, Tariq M, Swain D. In vitro propagation of some threatened plant species of India. Curr. Sci. 2018; 114(3):567-575.
Renu, Bhattacharya A, Singh R. Optimization of micropropagation from nodal segments of apple (Malus x domestica. Borkh) cultivars Golden Delicious and Red Fuji. Curr. J. Appl. Sci. Technol. 2018;31(3):1-9.
Thakur AK, Saraswat A, Srivastava DK. In vitro plant regeneration through direct organogenesis in Populus deltoids clone G48 from petiole explants. J. Pl. Biochem. Biotechnol. 2012;21(1):23-29.
Rosa YBCJ, Monte-Bello CC, Dornelas MC. In vitro organogenesis and efficient plant regeneration from root explants of Passiflora suberosa L. (Passifloraceae). In Vitro Cell. Dev. Biol. Pl. 2016;52:64-71.
Gonzalez MV, Rey M, Rodriguez R. Plant regeneration from petioles of Kiwifruit micro shoots. Hortcult. Sci. 1995;30(6): 1302-1303.
Akbas FA, Isikalan C, Namli S, Basaran D. Micropropagation of Kiwi fruit (Actinidia deliciosa). Intl. J. Agricult. Biol. 2007;9(3):489-493.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-497.
Omar MS, Hameed MK, Al-Rawi MS. Micropropagation of date palm (Phoenix dactylafera L.). In: Bajaj, Y. P. S. (ed.), Biotechnology in Agriculture and Foresty Vol. 18. High. Technology and Micropropagation II. Springer-Verlag, Berlin. 1992;471-492.
Caldas LS, Haridasan P, Ferreira ME. Rooting and acclimatization of micropropagated Gypsophila paniculata plants. Hortc. Braz. 1998;l(1):87-132.
Nowak B, Miczynski K, Hudy L. Sugar uptake and utilization during adventitious bud differentiation on in vitro leaf explant of Wegierka zwykla plum (Prunus domestica). Pl. Cell Tiss. Org. Cult. 2004;76(3):255-260.
Siwach P, Grover K, Gill AR. The influence of plant growth regulators explants nature and sucrose concentration on in vitro callus growth of Thevetia peruviana Schum. Asian J. Biotechnol. 2011; 3(3):280-292.
Pandey H, Bhojwani SS. Promotion of androgenesis in rice anther culture by substitution of sucrose with maltose and mannitol. Biol. Pl.. 1999;42:125-128.
Amiri S, Kazemitabar SK. Enhancement of callus induction and regeneration efficiency from embryo cultures of Datura stramonium by adjusting carbon sources and concentrations. Afr. J. Biotechnol. 2011;10(50):10101-10107.
Thangavel P, John Britto S, Senthilkumar SR. Adventitious shoot regeneration from leaf explants of the valuable medicinal herb Plectranthus barbatus Andrews. Afr. J. Biotechnol. 2011;10(43):8562‐8569.
Hamidoghli Y, Sharaf ARN, Zakizadeh H. Organogenesis from seedling derived leaf explants of primrose (Primula heterochroma Stapf.) as a scarce Iranian plant. Aus. J. Crop Sci. 2011;5(4):391-395.
Meng R, Chen THH, Finn CE, Li J. Improving in vitro plant regeneration from leaf and petiole explants of ‘Marion’ blackberry. Hortc. Sci. 2004;39(2):316-320.
Lo KH, Giles KL, Sawhney VK. Acquisition of competence for shoot regeneration in leaf discs of Saintpaulia ionantha × confusa hybrids (African violet) cultured in vitro. Pl. Cell Rep. 1997;16:416–420.
Taha HS, Hassan MM, El-Bahr MK. Micro propagation of some Egyptian date palm dry cultivars, 1- Maturation of somatic embryos. Arab. J. Biotechnol. 2007;10(2):333-340.
Muniran F, Bhore SJ, Shah FH. Micropropagation of Elaeis guineensis Jacq. ‘Dura’: comparison of three basal media for efficient regeneration. Indian J. Exp. Biol. 2008;46(1):79-82.
Placide R, Clement U, Fracoise U, Vedaste A. Comparative study of effects of table sugar, laboratory grade sucrose and mannitol on growth of banana plantlets under in vitro conditions. Rwanda J. Agri. Sci. 2012;28:76-83.
Meenakshi S, Shinde BN, Suprasanna P. Somatic embryogenesis from immature male flowers and molecular analysis of regenerated plants in banana “Lal Kela” (AAA). Fruit Ornamental Pl. Res. 2011;19(2):15-30.
Thakur R, Rao PS, Bapat VA. In vitro plant regeneration in Melia azedarach L. Pl. Cell Rep. 1998;18:127-131.
Aliou N, Mamadou SD, Dame N, Yaye Kene GD. In vitro regeneration of adult trees of Bambusa vulgaris. Afr. J. Biotechnol. 2006;5:1245-1248.
Vengadesan G, Ganapathi A, Anand RP, Anbazhagan VR. In vitro propagation of Acacia sinuata (Lour.) Merr. via cotyledonary nodes. Agro. For. Systems. 2002;55:9-15.
Nanda RM, Das P, Rout GR. In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annl. For. Sci. 2004;61:381-396.
Pospisilova J, Ticha, I, Kadlecek P, Haisel D, Plzakova S. Acclimatization of micropropagated plants to ex vitro conditions. Biol. Plant. 1999;42:481- 497.
Deb CR, Imchen T. An efficient in vitro hardening technique of tissue culture raised plants. Biotechnol. 2010;9(1):79-83.
Deb CR, Pongener A. A study on the use of low cost substrata against agar for non-symbiotic seed culture of Cymbidium iridioides D. Don. Aus. J. Crop Sci. 2013;7(5):642-649.
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