INDUCTION OF POTATOES (Solanum tuberosum L.) MICRO TUBER AT SOME COUMARIN CONCENTRATIONS AND INCUBATION TEMPERATURES
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
Page 1-12
Abstract
Potatoes are a priority horticultural crop to be developed because they can be used for food diversification. Increasing the production of micro tubers is needed for the provision of potato seeds. The purpose of this study was to obtain the interaction between coumarin concentration and incubation room temperature on the induction of potato micro tubers. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang from September 2019 to January 2020. The study was a two-factor factorial with 4 replications in a completely randomized design (CRD). The first factor is the concentration of coumarin which consists of 5 levels, namely 0, 25, 50, 75 and 100 mg / l. The second factor is the temperature of the incubation room which consists of 3 levels, namely: 20, 25 and 30 ° C. The data were analyzed by using the F test and followed by the Tukey’s test. The research data showed that there was an interaction between the application of several coumarin concentrations and the incubation room temperature to time of tubers emergence, the fastest tubers appeared 13.26 days after the addition of the tuber medium (DAT) at 100 ppm of coumarin and a temperature of 30 ° C. The best application of 100 ppm coumarin for time of tubers emergence, number of tuber and fresh weight of root. Incubation room temperature affected time of tubers emergence, but did not affect diameter of tuber, fresh weight of tuber and fresh weight of shoot.
Keywords:
- Coumarin
- temperature
- induction
- micro tubers
- potato
How to Cite
WARNITA, W., MAYERNI, R., KRISTINA, N., & NADILA, N. E. (2021). INDUCTION OF POTATOES (Solanum tuberosum L.) MICRO TUBER AT SOME COUMARIN CONCENTRATIONS AND INCUBATION TEMPERATURES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(33-34), 1-12. Retrieved from https://ikppress.org/index.php/PCBMB/article/view/6259
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Al-Wakeel SM, Gabr MM, Abu-El-Soud WM, Pious AM. Coumarin and salicylic acid activate resistance to Macrophomina phaseolina in Helianthus annuus. Acta Agronomica Hungarica. 2013;61(1):23–35.
Ahrabi F, Enteshari S, Moradshahi A. Allelopathic potential of para-hydroxybenzoic acid and coumarin on canola: Talaieh cultivar. Journal of Medicinal Plants Research. 2010;5:5104–5109.
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Borna RS, Hoque MI, Sarker RH. 2019. In vitro microtuber induction and regeneration of plantlets from microtuber discs of cultivated potato (Solanum tuberosum L.). Plant Tissue Cult. & Biotech. 2019; 29(1):63-72, (June)
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Sari, H.P., W. Warnita, and Indra Dwipa. 2019. Rhizobacteria and Coumarin Applications on Growth and Tuber Formation of Potato (Solanum tuberosum L.). J. Agron. Indonesia, 47 (2): 188-195
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Suharjo UKJ, Fahrurrozi, Sudjatmiko S. 2008. Encouraging the formation of micro potato tubers at high temperatures with the application of paclobutrazol, coumarin, CCC, and ancymidol. Proceedings of the National Potato Week Seminar. Lembang, Bandung, 22-23 August; 2008.
Stark KL, Love SM. Potato production system. California: Mcgraw Hill; 2003.
Wattimena GA. Development of quality potato propagules from superior potato cultivars to support increased potato production in Indonesia. Scientific Oration of Permanent Professor of Horticulture, Faculty of Agriculture, IPB, Bogor; 2000.
Otroshy M, Nazarian F, Struik PC. Effects of temperature fluctuation during in vitro phase on in vitro microtuber production in different cultivars of potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult. 2009;98(2):213-218.
Suharjo UKJBG, Murcitro T, Pamekas, Haryuni. In vitro induction of micro potato tubers at high temperature with multiple tuber promoter. Biogenesis. 2017;5(1):61 - 69.
Rykaczewska K. The impact of high temperature during growing season on potato cultivars with different responses to environmental stresses. American Journal of Plant Sciences. 2013;4:2386-93.
Hasni VUA, Barus FET, Sitepu RCB. Hutabarat. Response adding to the coumarin in to micro tuber production of potato plantlets (Solanum tuberosum L.) varieties of granola. Online Journal of Agroecotechnology: 2014;2(4):1552- 1562.
Kianmehr B, Parsa M, Otroshy M, Mohallati MN, Moradi K. Effect of plant growth regulators during in vitrophase of potato microtuber production Journal of Agricultural Technology. 2012;8(5):1745-59
Kusumaningrum IS. Evaluation of in vitro growth and micro tuber production of several potato clones (Solanum tuberosum L.) from crosses of Atlantic and Granola cultivars [thesis]. Bogor Agricutural University. Bogor; 2007.
Kawakami J, Iwama K. Effect of potato size on the growth and yield performance of field grown plants. Plant Prod. Sci. 2012; 15 (2):144-148.
Sumarni E, Suhardiyanto H, Saptomo SK. Root zone cooling in potato seed production using an aeroponic system. J. Agron. Indonesia. 2013;41:154-159.
Color. Effect of growth media and photoperiod on potato microtuberization. Journal of Agrosia Deed. 2007;10(2):167-171.
Suharjo UKJ, Hasanudin H, Pamekas T, Pujiwati H, Vanturini A. Promoting tuber formation in vitro with benzyl amino purine and paclobutrazol at different concentrations. Agrosia Deed. 2019;22(1): 29-35.
Weaver. Plant Growth Substances in Agriculture. Mc Millan Publishing Co Inc. New York; 1972.
Warnita, Akhir N, Vina. Growth response of two varieties chrysanthemum (Chrysanthemum sp.) on some media composition. International Journal on Advanced Science, Engineering and Information Technology. 2017;7(3):928-935.
Warnita. Study of seeding patterns of several potato genotypes in the field and in vitro in the effort to supply seeds. Andalas University Postgraduate Dissertation, Padang. 2006;185.
Hidayat MI, Sudjuko S. The technique of rapid propagation of potato seed tubers. Indonesian Vegetable Crops Research Institute. Bandung. 2016;28.
Saha S, Ahmed M, Islam MM, Reme RN, Ali MR. Effect of different levels of sucrose on microtuberization and different substrates on minituber production from potato meristem culture. IOSR Journal of Agriculture and Veterinary Science. 2013;4(6):58-62.
Saputra A. Effect of giving several coumarin concentrations on potato (Solanum tuberosum L.) rooting in vitro. Thesis Faculty of Agriculture; 2009.
Bodani A, Chauhan JS. In vitro sterilization protocol for micropropagation of Solanum tuberosum cv. Kufri Himalini. Academia Arena. 2010;2(4):24-27.
Murashige T. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 1974;25:135-166.
Gamborg OL, Shyluk JP. Nutrition, media and characteristics of plant cell and tissue culture, In Thorpe T.A. (Ed.) Plant Tissue Culture Methods Application in Agriculture. Academic Press Inc., New York. 1981;21-41.
Pierik RLM. In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, The Nederlands. 1987;344.
Elaleem KGA, Modawi RS, Khalafalla MM. Micro tuber Induction of two Potato (Solanum tuberosum L.) Varieties namely, Almera and Diamant. International Research Journal of Biological Sciences. 2015;4(3):84-89.
Suhadi I, Nurhidayati BA. Sharon. The effectiveness of synthetic retardants on the growth and longevity of sunflowers (Hellianthus annus L.). Journal of Agrifor. 2017;XIV(2):219-228.
Amalia IA. Nuraini ∙Sumadi ∙S. Mubarok E. Suminar. Formation of potato micro potato (Solanum tuberosum L.) on various media compositions in vitro. Cultivation Journal. 2017;16(3):389 – 393.
Sakya TA, Yunus A, Samanhudin U. Baroroh. The effect of coumarin and aspirin on induction of potato microtuber. Agrosciences. 2003;5(1):19-28.
Al-Wakeel SM, Gabr MM, Abu-El-Soud WM, Pious AM. Coumarin and salicylic acid activate resistance to Macrophomina phaseolina in Helianthus annuus. Acta Agronomica Hungarica. 2013;61(1):23–35.
Ahrabi F, Enteshari S, Moradshahi A. Allelopathic potential of para-hydroxybenzoic acid and coumarin on canola: Talaieh cultivar. Journal of Medicinal Plants Research. 2010;5:5104–5109.
Zhou X, Song H, Wang J. Effects of coumarin on net nitrate uptake and nitrogen metabolism in roots of alfalfa (Medicago sativa). Allelopathy Journal. 2013;31:377.
Warnita. Modification of potato rooting media with growth retardant substances. Straw Journal. 2008;1(1):50-52.
Hossain MS, Hossain MM, Haque MM, Haque MM, Sarkar MD. Varietal evaluation of potato microtuber and plantlet in seed tuber production. International Journal of Agronomy. 2017;Article ID 7520297.
DOI: 10.1155 / 2017/7520297
Borna RS, Hoque MI, Sarker RH. 2019. In vitro microtuber induction and regeneration of plantlets from microtuber discs of cultivated potato (Solanum tuberosum L.). Plant Tissue Cult. & Biotech. 2019; 29(1):63-72, (June)
Anisa, F. Effect of chitosan and coumarin on growth and yield of potato (Solanum tuberosum L.) G2 Cultivar Granola. Agri Journal SCI. 2014;1(4):Hal 100-110.
Sari, H.P., W. Warnita, and Indra Dwipa. 2019. Rhizobacteria and Coumarin Applications on Growth and Tuber Formation of Potato (Solanum tuberosum L.). J. Agron. Indonesia, 47 (2): 188-195
Dhaka M, Nailwal TK. High efficienty macropropagation of potato (Solanum tuberosum L.) cv. Kufru Jyoti in Kumaun Hills. J. Plant Breeding and Crop Sci. 2015; 7 (7):203-210.
Vural GE, Ozsan T, Gozen V, Onus AN. In vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?. International Journal of Biotech Trends and Technology (IJBTT). 2018;8(1):1-8.
Suharjo UKJ, Fahrurrozi, Sudjatmiko S. 2008. Encouraging the formation of micro potato tubers at high temperatures with the application of paclobutrazol, coumarin, CCC, and ancymidol. Proceedings of the National Potato Week Seminar. Lembang, Bandung, 22-23 August; 2008.
Stark KL, Love SM. Potato production system. California: Mcgraw Hill; 2003.
Wattimena GA. Development of quality potato propagules from superior potato cultivars to support increased potato production in Indonesia. Scientific Oration of Permanent Professor of Horticulture, Faculty of Agriculture, IPB, Bogor; 2000.
Otroshy M, Nazarian F, Struik PC. Effects of temperature fluctuation during in vitro phase on in vitro microtuber production in different cultivars of potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult. 2009;98(2):213-218.
Suharjo UKJBG, Murcitro T, Pamekas, Haryuni. In vitro induction of micro potato tubers at high temperature with multiple tuber promoter. Biogenesis. 2017;5(1):61 - 69.
Rykaczewska K. The impact of high temperature during growing season on potato cultivars with different responses to environmental stresses. American Journal of Plant Sciences. 2013;4:2386-93.
Hasni VUA, Barus FET, Sitepu RCB. Hutabarat. Response adding to the coumarin in to micro tuber production of potato plantlets (Solanum tuberosum L.) varieties of granola. Online Journal of Agroecotechnology: 2014;2(4):1552- 1562.
Kianmehr B, Parsa M, Otroshy M, Mohallati MN, Moradi K. Effect of plant growth regulators during in vitrophase of potato microtuber production Journal of Agricultural Technology. 2012;8(5):1745-59
Kusumaningrum IS. Evaluation of in vitro growth and micro tuber production of several potato clones (Solanum tuberosum L.) from crosses of Atlantic and Granola cultivars [thesis]. Bogor Agricutural University. Bogor; 2007.
Kawakami J, Iwama K. Effect of potato size on the growth and yield performance of field grown plants. Plant Prod. Sci. 2012; 15 (2):144-148.
Sumarni E, Suhardiyanto H, Saptomo SK. Root zone cooling in potato seed production using an aeroponic system. J. Agron. Indonesia. 2013;41:154-159.
Color. Effect of growth media and photoperiod on potato microtuberization. Journal of Agrosia Deed. 2007;10(2):167-171.
Suharjo UKJ, Hasanudin H, Pamekas T, Pujiwati H, Vanturini A. Promoting tuber formation in vitro with benzyl amino purine and paclobutrazol at different concentrations. Agrosia Deed. 2019;22(1): 29-35.
Weaver. Plant Growth Substances in Agriculture. Mc Millan Publishing Co Inc. New York; 1972.
Warnita, Akhir N, Vina. Growth response of two varieties chrysanthemum (Chrysanthemum sp.) on some media composition. International Journal on Advanced Science, Engineering and Information Technology. 2017;7(3):928-935.
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