THE EFFECT OF EXTRACTION METHOD ON THE CHEMICAL COMPOSITION OF Lamium amplexicaule .L ETHANOL EXTRACT DETERMINED BY HPLC/MS-MS
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 23, Issue 7-8,
Page 33-38
DOI:
10.56557/pcbmb/2022/v23i7-87458
Abstract
Lamium amplexicaule. L is a plant of the Lamiaceae family, widespread in Algeria and North Africa. In the literature, no detailed research elucidating the methods of extraction of phenolic compounds from this species is available. Therefore, the objective of this study was to evaluate the efficient method between cold and hot extraction conditions. Ethanolic extracts were obtained by maceration and Soxhlet apparatus. Phytochemical composition was determined by liquid chromatography mass spectrometry (LC-MS/MS) method. Results demonstrate that the major compound in both extracts was chlorogenic acid. However, 18 compounds were identified in the extract obtained by Soxhlet and 17 in that obtained by maceration. The quantification of these constituents showed higher amounts in the Soxhlet extract than the maceration one. Therefore, it can be concluded that the Soxhlet extraction is more efficient for L. amplexicaule than the maceration one.
Keywords:
- L. amplexicaule
- extraction
- maceration
- Soxhlet
- HPLC/MS
- polyphenols
How to Cite
SIHAM, F., & RACHID, B. (2022). THE EFFECT OF EXTRACTION METHOD ON THE CHEMICAL COMPOSITION OF Lamium amplexicaule .L ETHANOL EXTRACT DETERMINED BY HPLC/MS-MS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 23(7-8), 33–38. https://doi.org/10.56557/pcbmb/2022/v23i7-87458
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Sajjadi S E, Ghannadi A. Analysis of the essential oil of Lamium amplexicaule L. from Northeastern Iran. Journal of Essential Oil Bearing Plants. 2012;15(4):577–581.
El-Hela AA, Abdel-Hady NM, Dawoud GTM, Ghoneim MM. HPTLC fingerprint profile oftriterpenes of Lamium amplexicaulebenth. and Ajugaiva L. (Lamiaceae) monitored with screening of their anti-inflammatory effect. Journal of Pharmacognosy and Phytochemistry. 2016; 5(6):176-181.
Sakamoto C, Suzuki M, Iwasaki A, Suenaga K, Kato-Noguchi H. Evaluation of allelopathic competency of Lamium amplexicaule and identification of its allelopathic active substance.Emirates Journal of Food and Agriculture. 2019; 31(2):76-80
Erbil N, Alan Y, Digrak M. Antimicrobial and antioxidant properties of Lamiumgalactophyllum boiss & reuter, L. macrodon boiss & huet and L. amplexicaule from Turkish Flora. Asian Journal of Chemistry. 2014;26(2):549- 554.
Laggoune S, Boutaghane N, Kabouche A, Kabouche Z, Ait-Kaki Z, Ait-Kaki B. Components and antimicrobial activity of Lamium amplexicaule from Algeria. Chemistry of Natural Compounds. 2008; 44(3).
Ferdjioui S, Belhattab R, Al-Zoubi RM. Chemical composition and antioxidant effect of mentharotundifolia extracts. Pharmacogn J. 2019;11(3):521-526.
Sankeshwari RM, Ankola AV, Bhat K, Hullatt K. Soxhlet versus cold maceration: Which method gives better antimicrobialactivity to licorice extract against streptococcus mutans?. Journal of the Scientific Society. 2018;45:67 71.
Widyaningrum I, Wibisono N, Kusumawati AH. Effect of extraction method on antimicrobial activity against staphylococcus aureus of tapakliman (Elephantopusscaber l.) leaves. International Journal of Health & Medical Sciences. 2020;3(1):105-110.
Cacique AP, Barbosa ÉS, de Pinho GP, Silvério FO. Maceration extraction conditions for determining the phenolic compounds and the antioxidant activity of Catharanthus roseus (L.) G. Don. Ciênciae Agrotecnologia. 2020;44:e017420.
Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants. 2015;4(3):3-6.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale PharmaceuticaSciencia. 2011;1(1): 98-106.
Rasul GM. Conventional extraction methods use in medicinal plants, their advantages and disadvantages. International Journal of Basic Sciences and Applied Computing.2 (6). ISSN: 2394-367X;2018.
Golubović TD, Stojanović GS, KitićDv, Zlatković BK, Pavlović DR, Jovanović SČ, Lazarević JS. Comparative study of the ethanol extracts of six Acinos Miller species:chemical composition, antimicrobial and antioxidative activities. NotulaeBotanicaeHortiAgrobotanici Cluj-Napoca. 2020;48(1):53-65.
Hikmawanti NE, Fatmawati S, Asri AW. The effect of ethanol concentrations as the extraction solvent on antioxidant activity of katuk (Sauropus androgynus (L.)Merr.) leaves extracts. Earth and Environmental Science. 2021;755:012060.
Jung H, Im S, Song D, Bae J. Effects of chlorogenic acid on intracellular calcium regulation in lysophosphatidylcholine-treated endothelial cells. BMB Rep. 2017;50(6):323-328.
Xue N, Zhou Q, Ji M, Jin J, Lai F, Chen J, Zhang M, Jia J, Yang H, Zhang J, Li W, Jiang J, Chen X. Chlorogenic acid inhibits glioblastoma growth through repolarizating macrophage from M2 to M1 phenotype. Scientific Reports. 2017; 7:39011.
Bender O, Atalay A. Evaluation of anti-proliferative and cytotoxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening. Marmara Pharmaceutical Journal. 2018;22(2):173-179.
Habtemariam S. Molecular pharmacology of rosmarinic and salvianolic acids: Potential seeds for alzheimer’s and vascular dementia drugs. International Journal o f Molecular Sciences. 2018; 19:458;1-25.
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