AGRONOMIC FACTORS ASSOCIATED WITH STEM ROT OF BERSEEM (Trifolium alexanderinum L.) IN SARGODHA, PAKISTAN

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EJAZ ASHRAF
SALMAN AHMAD
MUHAMMAD ZEESHAN NADEEM
MALIK ABDUL REHMAN
MUHAMMAD ATIQ
YASIR ALI
FAZAL SAID
MUHAMMAD EHETISHAM UL HAQ
SAIMA NASEER
FAZEELA KAYNAT

Abstract

Stem rot of berseem (SRB) caused by Sclerotinia sclerotiorum (SS) is the most devastating and cosmopolitan plant disease, and infecting more than 400 species of plants worldwide. The disease is reported to cause more than $200 million losses. In Pakistan, the disease is infecting all cultivars of Berseem and causing heavy losses. The Sargodha district is the main Berseem cultivation area of Punjab, Pakistan.  Agronomic practices are playing key role in its spread and dissemination to new fields. The hypothesis of current study was that this disease may be curtailed by altering agronomic practices. Hence, the following study was planned on the objective to evaluate the effect of agronomic practices of district Sargodha farmers on the disease incidence (DI) of SRB. To accomplish this objective, a detailed survey was arranged in seven tehsils of Sargodha during Berseem season in 2020-2021. The surveys were repeated every fifteen days throughout the cropping season. The villages and fields were selected randomly during the surveys. Every field was divided into five sections to note down the DI of SS. Agronomic practices surveys showed significant effect on SRB incidence in different tehsils. However, the effect of agronomic practices was different in different tehsils of Sargodha. In tehsil Kotmomin and Sargodha, canal irrigations, four number of irrigations, citrus inter-cropping, October and September-October cropping seasons, respectively, significantly (P < 0.05) decreased DI of SRB. In tehsil Sargodha, canal irrigations, four number of irrigations, citrus inter-cropping, and October cropping season significantly (P < 0.05) decreased DI of SRB. In tehsil Shahpur, canal irrigations, six number of irrigations, September cropping season and citrus intercropping significantly (P < 0.05) decreased DI of SRB. In tehsil Sahiwal and Sillanwali, tube-well+ canal irrigations, four number of irrigations, mustard and citrus inter-cropping, respectively, and October cropping season significantly (P < 0.05) decreased DI. It was concluded that change in agronomic practices could help lessen the SRB incidence in district Sargodha. The current study sets the foundation of future study to further investigate the role of different agronomic factors to eco-friendly manage SRB.

Keywords:
Berseem, stem rot, intercropping, number of irrigations, type of irrigations, cropping seasons

Article Details

How to Cite
ASHRAF, E., AHMAD, S., NADEEM, M. Z., REHMAN, M. A., ATIQ, M., ALI, Y., SAID, F., HAQ, M. E. U., NASEER, S., & KAYNAT, F. (2022). AGRONOMIC FACTORS ASSOCIATED WITH STEM ROT OF BERSEEM (Trifolium alexanderinum L.) IN SARGODHA, PAKISTAN. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 23(17-18), 46-59. Retrieved from https://ikppress.org/index.php/PCBMB/article/view/7656
Section
Original Research Article

References

Dheer S, Joshi YP, Virpal S, Sachan HK. Chemical weed management in Berseem (Trifolium alexandrium L.). Pantnagar Journal of Research. 2010;8(1):5-7.

Rouhi HR, Aboutalebian MA, Moosavi SA, Karimi FA, Karimi F, Saman M, Samadi M. Change in several antioxidant enzymes activity of Berseem clover (Trifolium alexandrinum L.) by priming. International Journal of AgriScience. 2012;2(3):237-243.

Al-Suhaibani NA, Al-Doss AA. Evaluation of growth and forage production for six varieties of Egyptian clovers under Riyadh Conditions. Journal of King Saud University Agricultural Sciences. 2004;16(1):45-51.

Saeed B, Gul H, Wahab S, Durrani Y, Haleema B, Ayub M, Ahmad I. Effect of phosphorus and potassium on seed production of berseem. 2011. African Journal of Biotechnology. 2011;10(63): 13769-13772.

Sharma P, Meena PD, Chauhan JS. Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India. African Journal of Microbiology Research. 2013;7(18):1827-33.

Anwar MZ, Khan MA, Saeed I, Ali A, Zahid S, Majid A. Small farmers perceptions regarding improved fodder and forage varieties: Results of participatory on farm research. Pakistan Journal of Agriculture Research. 2012;25(4):295-306.

Purdy L. Sclerotinia sclerotiorum history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology. 1979;69(8):875-880.

Heffer LV, Johnson KB. White mold. The plant health instructor.

DOI: 10.1094. PHI-I-2007; 0809-01

McDonald JT, Kennedy S. Insights into the ‘healthy immigrant effect’: Health status and health service use of immigrants to Canada. Social Science and Medicine. 2004;59(8):1613-1627.

Twengström E, Köpmans E, Sigvald R, Svensson C. Influence of different irrigation regimes on carpogenic germination of sclerotia of sclevotinia sclerotiorum. Journal of Phytopatholog. 1998;146(10): 487-493.

Saharan GS, Mehta N. Sclerotinia diseases of crop plants: Biology, ecology and disease management. Springer Science & Business Media; 2008.

Yang M, Zhang W, Lv Z, Shi L, Zhang K, Ge B. Evaluation of the inhibitory effects of Wuyiencin, a secondary metabolite of Streptomyces albulus CK-15, against Sclerotinia sclerotiorum in vitro. Plant Disease. 2022;31;106(1):156-64.

Shahoveisi F, Riahi Manesh M, del Río Mendoza LE. Modeling risk of Sclerotinia sclerotiorum-induced disease development on canola and dry bean using machine learning algorithms. Scientific Reports. 2022;12(1):1-0.

Cao Y, Yan X, Ran S, Ralph J, Smith RA, Chen X, Qu C, Li J, Liu L. Knockout of the lignin pathway gene BnF5H decreases the S/G lignin compositional ratio and improves Sclerotinia sclerotiorum resistance in Brassica napus. Plant, Cell and Environment. 2022;1-19.

Bolton MD, Thomma BP, Nelson BD. Sclerotinia sclerotiorum (Lib.) de Bary: Biology and molecular traits of a cosmopolitan pathogen. Molecular Plant Pathology. 2006;7(1):1-16.

Maghazy SMN, Abdelzaher HMA, Haridy MS, Moustafa SMN. Biological control of damping-off disease of Trifolium alexandrinum L. caused by Pythium spinosum Sawada var. spinosum using some soil fungi. Archives of Phytopathology and Plant Protection. 2008;41(6):431-450.

Atri A, Cheema HK, Singh N. Ecofriendly management of stem rot of berseem caused by Sclerotinia trifoliorum. European Journal of Plant Pathology. 2021;160(3): 649-62.

Iqbal MF, Iqbal Z. Efficacy of fungicides sprayed against rottening of berseem. International Journal of Advanced Multidisciplinary Research. 2014;1(2):22- 4.

Kohn LM. Delimitation of the economically important plant pathogenic Sclerotinia species. Phytopathology. 1979;69:881-6.

Safdar A, Javed N, Khan SA, Khan HU, Rehman A, Haq IU. Survey and investigation of different citrus growing areas for citrus sudden death syndrome. Pakistan Journal of Phytopathology. 2010;22(2):71-78.

Ricker AJ, Ricker RS. Introduction to research and plant diseases. John Swift Co., New York. 1936; 10:177-183.

Alam MW, Rehman A, Malik A, Mehboob S, Sarwar M, Muhammad S. First report of white mould of potato caused by Sclerotinia sclerotiorum in Pakistan. Journal of Plant Pathology. 2021;103(2):669.

Steadman JR, Marcinkowska J, Rutledge S. A semi-selective medium for isolation of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology. 1994;16(1):68-70.

Choi IY, Kim J, Lee WH, Cho SE, Shin HD. First report of Sclerotinia stem rot caused by Sclerotinia sclerotiorum on Chinese chives in Korea. Plant Disease. 2017;101(11):1953-1953.

O’Sullivan CA, Belt K, Thatcher LF. Tackling control of a cosmopolitan phytopathogen: Sclerotinia. Frontiers in Plant Science. 2021;1764.

Mahesha HS, Keerthi MC, Manjunatha N, Singh T, Vinaykumar HD, Bhargavi HA, Yadav VK. First report of Sclerotium rolfsii causing collar rot on Berseem (Trifolium alexandrinum) in India. Plant Disease. 2021;105(4):1208.

Saira M, Rehman A, Gleason ML, Alam MW, Abbas MF, Ali S, Idrees M. First Report of Sclerotinia sclerotiorum causing stem and crown rot of Berseem (Trifolium alexandrinum) in Pakistan. Plant Disease. 2017;101(5):835.

Chaudhry AR, Haq I, Rehman N. A cultural approach towards control of berseem root rot. Pakistan Journal of Agricultural Sciences. 1992;29(1):65-68.

Mueller DS, Pedersen WL, Hartman GL. Effect of crop rotation and tillage system on Sclerotinia stem rot on soybean. Canadian Journal of Plant Pathology. 2002;24(4):450-456.

Garza JG, Neumann S, Vyn TJ, Boland GJ. Influence of crop rotation and tillage on production of apothecia by Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology. 2002;24(2):137-143.

Reis A, Nascimento WM. New apiaceous hosts of Sclerotinia sclerotiorum in the Cerrado region of Brazil. Horticultura Brasileira. 2011;29(1):122-4.

Garg H, Sivasithamparam K, Banga SS, Barbetti MJ. Cotyledon assay as a rapid and reliable method of screening for resistance against Sclerotinia sclerotiorum in Brassica napus genotypes. Australasian Plant Pathology. 2008;37(2):106-11.

Steel RG. Analysis of variance II: Multiway classifications. Principles and procedures of statistics: A Biometrical Approach. 1997;204-52.

Maina MM, Zakari MD. Irrigation water quality assessment for soil nutrients content at Tanjungkarang irrigation scheme Malaysia. Bayero Journal of Engineering and Technology. 2017;12(2):61-68.

Korus KA, Lang JM, Adesemoye AO, Block CC, Pal N, Leach JE, Jackson-Ziems TA. First report of Xanthomonas vasicola causing bacterial leaf streak on corn in the United States. Plant Disease. 2017;101:1030.

Duthie JA. Models of the response of foliar parasites to the combined effects of temperature and duration of wetness. Phytopathology. 1997;87(11):1088-95.

Wen L, Tan TL, Shu JB, Chen Y, Liu Y, Yang ZF, Zhang QP, Yin MZ, Tao J, Guan CY. Using proteomic analysis to find the proteins involved in resistance against Sclerotinia sclerotiorum in adult Brassica napus. European journal of plant pathology. 2013;137(3):505-23.

Khan MA, Cowling W, Banga SS, You MP, Tyagi V, Bharti B, Barbetti MJ. Patterns of inheritance for cotyledon resistance against Sclerotinia sclerotiorum in Brassica napus. Euphytica. 2020;216(5):1-1.

Willbur JF, Fall ML, Bloomingdale C, Byrne AM, Chapman SA, Isard SA, Magarey RD, McCaghey MM, Mueller BD, Russo JM, Schlegel J. Weather-based models for assessing the risk of Sclerotinia sclerotiorum apothecial presence in soybean (Glycine max) fields. Plant Disease. 2018;102(1):73-84.