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Medicinal plants have served as the basis of alternative medicine and have become the primary source for developing new medications. Astragalus spinosus (Forssk.) Muschl. belongs to family Fabaceae and due to its active constituent’s composition, it is used traditionally to treat various ailments. Additionally, various Astragalus-based dietary supplements were sold for supporting immunity and cardiovascular system. Therefore, authentication of the medicinal plant material is important and vital to provide an extensive database with DNA data for easy identification, adulteration detection and conservation of genetic resources. In the current study, three partial chloroplast coding genes were examined in A.spinosus; ribulose 1,5biphosphate carboxylase large subunit (rbcL), RNA polymerase (rpoC1) and maturase K (matK). When compared to the National Center for Biotechnology Information (NCBI) database, the results showed that rbcL was successful in identifying and discriminating A.spinosus at the genus and species levels. The, rpoC1 and matK genes revealed a high level of identity with Astragalus species, indicating that they are capable of identifying A.spinosus at the genus level. Phylogenetic trees were established based on the expected amino acids sequence of each coding gene, demonstrating the extent of relatedness with recorded plant species in the NCBI database.
Helton LR. Folk medicine and health beliefs: An Appalachian perspective. J. Cult. Divers. 1996;3:123-128.
“List of plants in the family Fabaceae” Encyclopedia Britannica.
Retrived 28 April, 2021.
Abd El-Ghani MM, El-Sayed ASA, Moubarak A, Rashad R, Nosier H, Khattab A. Biosystematic Study on some Egyptian species of Astragalus L. (Fabaceae). Agriculture. 2021;11:125.
Lock M, Schrire BD, Galegeae. In Legumes of the world, Lewis, G.P., Schrire, B.D., Mackinder, B.A. and Lock, M., Eds.; Royal Botanic Gardens: Kew, India. 2005;475-481.
Rizk AM. The phytochemistry of the flora of Qatar. Scientific and Applied Research Centre, University of Qatar, Qatar. 1986;50-150.
Riaz Ullah, Alqahtani AS, Noman OMA, Alqahtani AM, Ibenmoussa S, Bourhia M. A review on ethno-medicinal plants used in traditional medicine in the Kingdom of Saudi Arabia. Saudi Journal of Biological Sciences. 2020;27:2706–2718.
Bedir E, Pugh N, Calis I, Pasco DS, Khan IA. Immunostimulatory effects of cycloartane-type triterpene glycosides from Astragalus species. Biol. Pharm. Bull. 2000; 23(7):834-837.
Tackholm V. Students flora of Egypt. 2nd Edn. Cairo University Cooperative Printing Co., Beirut; 1974.
Darwish F. Phytochemical investigation of biologically active fractions of Astragalus spinosus roots grown in Egypt. J. Med. Sci. 2002;2(3):119-123.
Essawy AE, Abd Elkader HA, Khamiss OA, Eweda SM, Abdou HM. Therapeutic effects of astragaloside IV and Astragalus spinosus saponins against bisphenol A-induced neurotoxicity and DNA damage in rats. Peer J. 2021;9:e11930.
Aboul-Enein A, Shalaby E, El-Shemy H. Traditional medicinal plants research in Egypt: Studies of antioxidant and anticancer activities. Journal of Medicinal Plants Research. 2012;6(5):689-703.
Ahmed, M. and Al-Dousari, N. (2020). The environmental and economic importance of Astragalus Spinosus in land restoration. Journal of Taibah University for Science, Vol. 14 (1):1489–1495.
Hebert PDN, Cywinska A, Ball SL, deWaard JR. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences. 2003;270:313–321.
Kress WJ. Plant DNA barcodes: Applications today and in the future. Journal of Systematics and Evolution. 2017; 55(4):291–307.
Dong W, Liu J, Yu J, Wang L, Zhou S. Highly variable chloroplast markers for evaluating plant phylogeny at low taxonomic levels and for DNA barcoding. PLoS ONE. 2012;7:e35071.
Khan S, Al-Qurainy F, Nadeem M, Tarroum M. Selection of chloroplast DNA markers for the development of DNA barcode and reconstruction of phylogeny of senecio asirensis boulos and jri wood. Pak. J. Bot. 2013;45:703-710.
Peterson PM, Romaschenko K, Johnson G. A classification of the Chloridoideae (Poaceae) based on multi-gene phylogenetic trees. Mol. Phylogenet Evol. 2010;55:580-598.
Vijayan K, Tsou CH. DNA barcoding in plants: Taxonomy in a new perspective. Current Science. 2010;99:1530–1541.
Retrieved from https://www.jstor.org/stable/24069450.
Techen N, Parveen I, Pan Z, Khan IA. DNA barcoding of medicinal plant material for identification. Current Opinion in Biotechnology. 2014;25:103–110.
Kress WJ, Erickson DL. A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE; 2007.
Hollingsworth PM, Forrest LL, Spouge JL, Little DP, et al. A DNA barcode for land plants. PNAS. 2009;106(31):12794–12797.
Udensi OU, Ita EE, Ikpeme EV, Ubi G, Emeagi LI. sequence analysis of maturase k (matk): A chloroplast-encoding gene in some selected pulses. Global Journal of pure and Applied Sciences. 2017;23:213-230.
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 2016;33:1870-1874.
Elsherif N, Ibrahim M. Implications of rbcL and rpoC1 DNA barcoding in phylogenetic relationships of some egyptian Medicago sativa L. Cultivars. Egypt. J. Bot. 2020;60(2):451-460.
Alkaraki AK, Aldmoor MA, Lahham JN, Awad M. DNA Barcoding of two thymelaeaceae species: Daphne mucronata royle and Thymelaea hirsuta (L.) Endl. Plants. 2021;10:2199.
Zahra NB, Shinwari ZK, Qaiser M. DNA barcoding: A tool for standardization of herbal medicinal products (hmps) of Lamiaceae from Pakistan Pak. J. Bot. 2016; 48(5):2167-2174.
Tallei TE, Kolondam BJ. DNA Barcoding of sangihe nutmeg (Myristica fragrans) using matK gene. HAYATI Journal of Biosciences. 2015;22(1):41-47.
Girma G, Spillane C, Gedil M. DNA barcoding of the main cultivated yams and selected wild species in the genus Dioscorea. Journal of Systematics and Evolution. 2015;54(3):228–237.
Liu G, Ning H, Ayidaerhan N, Aisa HA. Evaluation of DNA barcode candidates for the discrimination of Artemisia L. Mitochondrial DNA Part A, DNA Mapping, Sequencing, and Analysis. 2017;28(6):956-964.
Palhares RM, Gonçalves DM, Alves BS, Cosenza GP, Brandão MG, Oliveira G. Medicinal plants recommended by the world health organization: DNA barcode identification associated with chemical analyses guarantees their quality. PLoS ONE. 2015;10(5):e0127866.
Subedi A, Kunwar BB, Choi Y, Gravendeel B, et al. Collection and trade of wild-harvest orchids in Nepal. Journal of Ethnobiology and Ethnomedicine. 2013;9(1):64.
Huang W, Li F, Liu Y, Long C. Identification of Crocus sativus and its adulterants from chinese markets by using DNA barcoding technique. Iranian Journal of Biotechnology. 2015;13(1):e1034.
Williamson J, Maurin O, Shiba SNS, Bank HV, Pfab M, Pilusa M, Kabongo RM, Bank MV. Exposing the illegal trade in cycad species (Cycadophyta: Encephalartos) at two traditional medicine markets in South Africa using DNA barcoding. Genome. 2016;59(9).
Yu J, Wu X, Liu C, Newmaster S, Ragupathy S, Kress WJ. Progress in the use of DNA barcodes in the identification and classification of medicinal plants. Ecotoxicology and Environmental Safety. 2021;208:111691.