TRIPLE TEST CROSS ANALYSIS FOR DETERMINING GENE ACTION, GENETIC CORRELATIONS AND PREDICTION FOR YIELD AND YIELD COMPONENTS IN BREAD WHEAT

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A. A. KANDIL
M. A. ABDEL-MONEAM
H. HAFEZ, SOAD
S. GOMAA HASNAA

Abstract

In two crosses of bread wheat (Gemmeiza11 x Misr1 and Gemmeiza12 x Misr2) to investigate gene action, genetic correlations and predicating new recombinant lines for days to heading, plant height, flag leaf area, number of spikes/plant, number of grains/spike, 1000-grain weight, spike grain weight and grain yield /plant, triple test cross was used. The starting materials for triple test cross was the F1 and their grand parents (P1 and P2) for the two studied wheat crosses. The first cross Gemmeiza 11 x Misr1 and the second cross was Gemmeiza 12x Misr2. The F1’s were selfed to produce F2’s grains. In order to create F1 and F2 plants, the obtained materials (F1 and F2 grain genotypes) and the parental genotypes were seeded together. Thirty individual F2 plants were randomly labeled from each cross and crossed back to their grand parents (P1 and P2) and F1 between then to produce three types of families L1 (F2i x P1i), L2 (F2i x P2i) and L3 (F2i x F1i) in each cross. On November 10, 2018, during the winter growing season, the triple test cross families (L1, L2 and L3), along with the parents, F1 and F2 in each cross, were seeded. Results showed that in the genetic system for the investigated features for two crosses, epistatic gene effects were significant. The types of epistasis (additive x additive, additive x dominance and dominance x dominance) were significant for all studied characters. For the majority of traits in both crossings, both additive and dominant genetic components were significant and involved in the genetic system. The average level of dominance for spikes/plant in cross 1 and spike grain weight in cross -2 was overdominance, while for the remaining characters, additive gene effects were more pronounced. The F value was positive and significant for 1000-grain weight in two crosses, suggesting that dominant genes controlling these characters were unidirectional. The highest proportion of inbreds excepted to outperform parental rang in cross 1 for days to heading and flag leaf area. In both crosses, grain yield per plant was positively and significantly correlated with number of spikes per plant, number of grains per spike, and 1000-grain weight, according to epistasis, additive, and dominance genetic correlations.

Keywords:
Hypridization, triple test crossc, gene expression, gene action, additive gene effect, epestasis gene effect

Article Details

How to Cite
KANDIL, A. A., ABDEL-MONEAM, M. A., SOAD, H. H., & HASNAA, S. G. (2022). TRIPLE TEST CROSS ANALYSIS FOR DETERMINING GENE ACTION, GENETIC CORRELATIONS AND PREDICTION FOR YIELD AND YIELD COMPONENTS IN BREAD WHEAT. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 23(39-40), 47-57. Retrieved from https://ikppress.org/index.php/PCBMB/article/view/8013
Section
Original Research Article

References

Kearsey MJ, Jinks JL. A general method of detecting additive, dominance and epistatic variation for metrical traits Heredity. 1968; 23:403–409.

Rajesh Y, Behl RK, Yadav R. Genetics of morpho-physiological characters and grain yield in wheat. National J. Plant Improvement. 2003;4(2):26-29.

Nanda GSP, Singh and Gill KS. Estimating epistasis through triple test cross in wheat Indian J. Genet. 1983;43:160-163.

Iqbal Singh, Pawar IS, Singh S. Detection of genotype x environment interaction in spring wheat through triple test cross analysis. Crop. Improv. 1989;16:34 – 37.

Eissa MM. Triple test cross analysis in bread wheat (Triticum aestivum L.). Zagazig J. Agric. Res. 1994a;21:1-10.

Eissa MM. Detecting epistasis for yield and its components in wheat using triple test cross analysis (Triticum aestivum L.). Zagazig J. Agric. Res. 1994b;21:11- 20.

Eissa MM. Genetic correlation and predicting new recombinant lines in bread wheat using triple test cross analysis, Zagazig J. Agric. Res. 1994c;21:12-31.

Singh G. Estimation of gene action through triple test cross in bread wheat. Indian J. of Agric. Sci. 1989;59:700 – 702.

Katiyar PK, Ziauddin A. Detection of epistasis components over two environments in bread wheat Indian J. Genet. 1996;56:285-291.

Al-Kaddoussi AR. Using genetic components for predicting new recombinant lines in some crosses of Egyptian wheat (Triticum aestivum L.). Zagazig J. Agric. Res 1996;23:463-475.

Salama SM, Awaad SA, Manal M. Salem. Estimates of genetic components, prediction and genetic correlation in wheat (Triticum aestivum L.). Using North Carolina Design III J. Agric., Sci., Ain Shams Univ Cario. 2006;14:265-280.

Jinks JL, Perkins JM. A general method for the detecting of additive, dominance and epistatic components of variations. III F2 and backcrosses populations. Heredity. 1970;25:419 – 429.

Shahid N, Khan AS, Zuflqar A. Combining ability analysis for yield and yield contributing traits in bread wheat. J. of Agri. Social Sciences. 2005;2:129- 132.

Comstock RF, Robinson HF. Estimation of average Dominance of Genes. Heterosis, Chap. 30. Lowa State College Press; 1952.

Darwish MAH. Genetic studies on some physiological and agronomic characters for some bread wheat crosses. M.Sc. Thesis, Kafr EL-Shekh, Univ., Egypt; 2007.

Jinks JL, Perkins JM, Breese EL. A general method of detecting additive, dominance and epistasis variation for metrical traits: 11 Application to inbred lines Heredity. 1969;24:45-57.

Jinks JL, Pooni HS. Predicting the properties of recopmbinant lines derived by signle seed descent. Heredity. 1976;36:253-266.

Pooni HS, Jinks JL. Predicting the properties of recombinant inbred lines derived by single seed descent for two or more characters simultanously. Heredity. 1978;40:349-361.

Toledo JFF, Pooni HS, Jinks JL. Predicting the properties of second cycle hybrids produced by intercrossing random sample of recombinant inbred lines. Heredity. 1984;53:285-292.

Hayward MD, Bosemark and Romagosa NO. Plant Breeding. Principles and Prospects. 1st ed. Chapman and Hall. London; 1993.

Abd El-Rahman, Magada E. Genetic analysis of yield, yield components and earliness in some bread wheat crosses. Egypt. J. Agric. Res. 2008;86(2):575 – 584.

Tousi Mojarrad M, Ghannadha MR. Diallel Analysis for Estimation of Genetic Parameters in Relation to Traits of Wheat Height in Normal and Drought ConditionsJ. of Sci. and Technology of Agric. and Natural Resources. 2008;12:143-156.

Kearsey MJ, Hayward MD, Devey FD, Arcionic S, Eggleston MP, Eissa MM. Genetical analysis of production characters in Lolium. I. Triple test cross analysis of spaced plant Performance. Theor. Appl. Genet. 1987;75:66-75.

Hassan M, Munir MY, Mujahid Kisana NS, Akram Z, Nazeer AW. Genetic Analysis of Some Biometric Characters in Bread Wheat (Triticum aestivum L.). J. of Biological Sci., 2004;4(4):480-485.

Jensen NF. A diallel selective mating system of cereal breeding crop Sci. 1970;10:629-635.

Singh S, Singh RB. Triple test cross analysis in two wheat crossess Heredity. 1976;37:173 – 177.

Al-Kaddoussi AR. Testing for epistasis, prediction and genetic correlation using north carolina Design III biometrical approach for Egyptian bread wheat (Triticum aestivum L.). Zagazig J. Agric. Res. 1997;24:37-50.

Ketata H, Smith LH, Edwards, MeNew RW. Detection of epistatic, additive and dominance variation in winter wheat (Triticum aestivum L. Thell). Crop. Sci. 1976;16:1-4.