Molecular Variability in Phaeoisariopsis personata Causing Late Tikka Leaf Spot of Ground Nut in Cuddalore District of Tamil Nadu
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 24, Issue 1-2,
Page 23-29
DOI:
10.56557/pcbmb/2023/v24i1-28147
Abstract
An experiment was carried out at Department of plant pathology, Annamalai University, Tamil Nadu, India. To test the five isolates of Phaeoisariopsis personata from different regions of groundnut Vridhachalam-2 (VRI-2) variety that showed typical symptoms of late tikka leaf spot disease from Cuddalore district viz., Shivapuri (LT1), Srimushnam (LT2), Chaithiram (LT3), Parangipettai (LT4), and Kammapuram (LT5) were collected. Random Amplified Polymorphic DNA (RAPD) analysis was used to detect the variations among the isolates of P. personata. Pair wise genetic similarities between the different isolates of P. personata were estimated by DICE similarity coefficient. The Dendrogram results revealed that these five isolates showed 66 per cent to 80 per cent similarity coefficient. There is no congruence between the RAPD pattern and the virulence pattern of test isolates. The clustering in the RAPD dendrogram was not associated with the virulence.
Keywords:
- Groundnut
- random amplified polymorphic DNA
- Phaeoisariopsis personata
- leaf spot
How to Cite
Sundaramoorthy, S., & Ivin, J. J. S. (2023). Molecular Variability in Phaeoisariopsis personata Causing Late Tikka Leaf Spot of Ground Nut in Cuddalore District of Tamil Nadu. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 24(1-2), 23–29. https://doi.org/10.56557/pcbmb/2023/v24i1-28147
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Paulraj PS, Renu G, Ranganathan K, Veeramanoharan R, Kumar A. Ectoparasites diversity on rodents and shrews at scrub typhus endemic Vellore District of Tamil Nadu, India. Journal of Arthropod-Borne Diseases. 2022;16(1):51-60.
Giordano DF, Pastor N, Palacios S, Oddino CM, Torres AM. Peanut leaf spot caused by Nothopassalora personata. Tropical Plant Pathology. 2021;46:139-151.
Roshania RP, Mehta RV, Shete A, Bingewar R, Kulkarni S, Mahajan A, Martorell R. Agreement between dried blood spots and HemoCue in Tamil Nadu, India. Scientific Reports. 2021;11(1):9285.
Palombi M, Damiano C. Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Reports. 2002;20:1061-1066.
Khedikar YP, Gowda MVC, Sarvamangala C, Patgar KV, Upadhyaya HD, Varshney RK. A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut (Arachis hypogaea L.). Theoretical and applied genetics. 2010;121:971-984.
Ghorpade BB, Salokhe SS, Ghuge VG, Adsul AT. Molecular characterization of different soybean [Glycine max (L). Merril] genotypes by RAPD Markers. Journal of Biotechnology & Bioinformatics Research. 2022;156(4):10-15.
Rampuria S, Bag P, Rogan CJ, Sharma A, Gassmann W, Kirti PB. Pathogen-induced AdDjSKI of the wild peanut, Arachis diogoi, potentiates tolerance of multiple stresses in E. coli and tobacco. Plant Science. 2018;272:62-74.
Patil AA, Velayudhan A, Durairaj GK, Khasnobis P, Sodha SV. Outbreak investigation of foodborne illness among political rally attendees, Cuddalore, Tamil Nadu, India. Indian Journal of Public Health. 2021;65(5):55.
Velayudhan A, Nayak J, Murhekar MV, Dikid T, Sodha SV. Shellfish poisoning outbreaks in Cuddalore District, Tamil Nadu, India. Indian Journal of Public Health. 2021;65(5):29.
Ayyandurai R, Venkateswaran S, Karunanidhi D. Hydrogeochemical assessment of groundwater quality and suitability for irrigation in the coastal part of Cuddalore district, Tamil Nadu, India. Marine Pollution Bulletin. 2022;174: 113258.
Ibny FY, Jaiswal SK, Mohammed M, Dakora FD. Symbiotic effectiveness and ecologically adaptive traits of native rhizobial symbionts of Bambara groundnut (Vigna subterranea L. Verdc.) in Africa and their relationship with phylogeny. Scientific reports. 2019;9(1):1-17.
Ganeshamoorthi P, Anand T, Saravanan A, Prakasam V, Chandramohan N, Ragupathi N. Cultural and genetic variability in Macrophomina phaseolina (Tassi.) Goid. and incidence of mulberry root rot. Archives of Phytopathology and Plant Protection. 2010;43(2):123-132.
Bhupathi P, Theradimani, M. Studied on pathogen variability in black gram root rot by Macrophomina phaseolina. Pharma Inno. 2018;7:3638.
Kharde RR, Lavale SA, Ghorpade BB. Molecular diversity among the isolates of Xanthomonas axonopodis pv. citri causing bacterial canker in citrus. Int.J. Curr. Microbiol. Appl. Sci. 2018;7:2375-2384.
Nagendran K, Mohankumar S, Mohammed Faisal P, Bagewadi B, Karthikeyan, G. Molecular evidence for the occurrence of tomato leaf curl New Delhi virus on chayote (Sechium edule) in southern India. Virus disease. 2017;28:425-429.
Valerio HM, Resende MA, Weikert-Oliveira RCB, Casela CR. Virulence and molecular diversity in Colletotrichum graminicola from Brazil. Mycopathologia. 2005;159: 449-459.
Sharma PN, Padder BA, Sharma OP, Pathania A, Sharma P. Pathological and molecular diversity in Colletotrichum lindemuthianum (bean anthracnose) across Himachal Pradesh, a north-western Himalayan state of India. Australasian Plant Pathology. 2007;36(2):191-197.
Ndegwa E, O’Brien D, Matthew K, Wang Z, Kim J. Shiga toxin subtypes, serogroups, phylogroups, RAPD genotypic diversity, and select virulence markers of shiga-toxigenic escherichia coli strains from goats in mid-atlantic US. Microorganisms. 2022;10(9):1842.
Christopher DJ, Raj TS, Kumar R. Morphological, pathological and molecular variability of Colletotrichum capsici causing anthracnose of chilli. Annals of Plant Protection Sciences, 2013;21(2): 309-314.
Fry WE, Birch PRJ, Judelson HS, Grünwald NJ, Danies G, Everts KL, Smart CD. Five reasons to consider Phytophthora infestans a reemerging pathogen. Phytopathology. 2015;105(7):966-981.
Thiessen LD, Woodward JE. Diseases of peanut caused by soilborne pathogens in the Southwestern United States. International Scholarly Research Notices; 2012.
Sharma A, Kumar D, Kumar S, Rampuria S, Reddy AR, Kirti PB. Ectopic expression of an atypical hydrophobic group 5 LEA protein from wild peanut, Arachis diogoi confers abiotic stress tolerance in tobacco. PloS one. 2016;11(3):e0150609.
Saha S, Ishaque NM, Burgsdorf I, Macho M, Ewe D, Steindler L, Saurav K. Draft genome sequence of terrestrial Streptomyces sp. strain VITNK9, isolated from Vellore, Tamil Nadu, India, exhibiting antagonistic activity against fish pathogens. Microbiology Resource Announcements. 2021;10(1):e01282-20.
Radhakrishnan T, Kona P, Ajay BC, Kumar N. Groundnut breeding. in fundamentals of field crop breeding Singapore: Springer nature Singapore. 2022;837-906.
Shinde VS, Raghuwanshi KS, Suryawanshi AV, Naik RM. Biochemical characterization and responses of resistant and susceptible groundnut genotypes to late leaf spot (Phaeoisariopsis personata (Berk. and Curt.) Von Arx.). Journal of Pure and Applied Microbiology. 2016; 10(4):3141-3150.
Thangavelu R, Gopi M, Pushpakanth P, Loganathan M, Edwin Raj E, Marimuthu, N, Uma SFirst Report of Fusarium oxysporum f. sp. cubense VCG 0125 and VCG 01220 of Race 1 Infecting Cavendish Bananas (Musa sp. AAA) in India. Plant Disease. 2021;105(4):1215-1215.
Nutsugah SK, Oti-Boateng C, Tsigbey FK, Brandenburg RL. Assessment of yield losses due to early and late leaf spots of groundnut (Arachis hypogaea L.). Ghana Journal of Agricultural Science. 2007; 40(1):21-27.
Pandya U, Saraf M. Integrated diseases management in groundnut for sustainable productivity. Bacteria in Agrobiology: Crop Productivity. 2013;351-377.
Fulmer AM, Mehra LK, Kemerait JRRC, Brenneman TB, Culbreath AK, Stevenson KL, Cantonwine EG. Relating Peanut Rx risk factors to epidemics of early and late leaf spot of peanut. Plant disease. 2019;103(12):3226-3233.
Culbreath AK, Kemerait JRRC, Brenneman TB, Cantonwine EG, Rucker KS. Effect of in-furrow application of fluopyram on leaf spot diseases of peanut. Plant Disease. 2021;105(9):2374-2379.
Jordan BS, Culbreath AK, Brenneman TB, Kemerait JRRC, Stevenson KL. Effect of planting date and peanut cultivar on epidemics of late leaf spot in Georgia. Plant disease. 2019;103(5):990-995.
Cai L, Hyde KD, Taylor PWJ, Weir B, Waller J, Abang MM, Shivas RG. A polyphasic approach for studying Colletotrichum. Fungal Diversity. 2009;39(1):183-204.
Vinothkannan A, Rajaram R, Charles PE, Ganeshkumar A. Metal-associated human health risk assessment due to consumption of pelagic and benthic ichthyofaunal resources from the highly contaminated Cuddalore coast in Southern India. Marine Pollution Bulletin. 2022; 176:113456.
Vinothkannan A, Charles PE, Rajaram R. Consumption of metal-contaminated shellfish from the Cuddalore coast in Southeastern India poses a hazard to public health. Marine Pollution Bulletin. 2022;181:113827.
Sivaramakrishnan S, Kannan S, Singh SD. Genetic variability of Fusarium wilt pathogen isolates of chickpea (Cicer arietinum L.) assessed by molecular markers. Mycopathologia. 2002;155(3): 171-178.
Anco DJ, Thomas JS, Wright DL, Dufault NS, Small IM. Sixty-one years following registration, phorate applied in-furrow at planting suppresses development of late leaf spot on peanut. Plant Disease. 2020;104(11):2885-2890.
Munir M, Wang H, Dufault NS, Anco DJ. Early detection of airborne inoculum of Nothopassalora personata in spore trap samples from peanut fields using quantitative PCR. Plants. 2020;9(10):1327.
Subramanian S, Jambulingam P, Krishnamoorthy K, Sivagnaname N, Sadanandane C, Vasuki V, Raju HKK. Molecular xenomonitoring as a post-MDA surveillance tool for global programme to eliminate lymphatic filariasis: Field validation in an evaluation unit in India. PLoS neglected tropical diseases. 2020; 14(1):e0007862.
George GM, Daniel HDJ, Mathew L, Peter D, George L, Pulimood S, Mammen S. Changing epidemiology of human enteroviruses (HEV) in a hand, foot and mouth disease outbreak in Vellore, south India. Indian Journal of Medical Microbiology. 2022;40(3):394-398.
Paulraj PS, Renu G, Ranganathan K, Veeramanoharan R, Kumar A. Ectoparasites diversity on rodents and shrews at scrub typhus endemic Vellore District of Tamil Nadu, India. Journal of Arthropod-Borne Diseases. 2022;16(1):51-60.
Giordano DF, Pastor N, Palacios S, Oddino CM, Torres AM. Peanut leaf spot caused by Nothopassalora personata. Tropical Plant Pathology. 2021;46:139-151.
Roshania RP, Mehta RV, Shete A, Bingewar R, Kulkarni S, Mahajan A, Martorell R. Agreement between dried blood spots and HemoCue in Tamil Nadu, India. Scientific Reports. 2021;11(1):9285.
Palombi M, Damiano C. Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Reports. 2002;20:1061-1066.
Khedikar YP, Gowda MVC, Sarvamangala C, Patgar KV, Upadhyaya HD, Varshney RK. A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut (Arachis hypogaea L.). Theoretical and applied genetics. 2010;121:971-984.
Ghorpade BB, Salokhe SS, Ghuge VG, Adsul AT. Molecular characterization of different soybean [Glycine max (L). Merril] genotypes by RAPD Markers. Journal of Biotechnology & Bioinformatics Research. 2022;156(4):10-15.
Rampuria S, Bag P, Rogan CJ, Sharma A, Gassmann W, Kirti PB. Pathogen-induced AdDjSKI of the wild peanut, Arachis diogoi, potentiates tolerance of multiple stresses in E. coli and tobacco. Plant Science. 2018;272:62-74.
Patil AA, Velayudhan A, Durairaj GK, Khasnobis P, Sodha SV. Outbreak investigation of foodborne illness among political rally attendees, Cuddalore, Tamil Nadu, India. Indian Journal of Public Health. 2021;65(5):55.
Velayudhan A, Nayak J, Murhekar MV, Dikid T, Sodha SV. Shellfish poisoning outbreaks in Cuddalore District, Tamil Nadu, India. Indian Journal of Public Health. 2021;65(5):29.
Ayyandurai R, Venkateswaran S, Karunanidhi D. Hydrogeochemical assessment of groundwater quality and suitability for irrigation in the coastal part of Cuddalore district, Tamil Nadu, India. Marine Pollution Bulletin. 2022;174: 113258.
Ibny FY, Jaiswal SK, Mohammed M, Dakora FD. Symbiotic effectiveness and ecologically adaptive traits of native rhizobial symbionts of Bambara groundnut (Vigna subterranea L. Verdc.) in Africa and their relationship with phylogeny. Scientific reports. 2019;9(1):1-17.
Ganeshamoorthi P, Anand T, Saravanan A, Prakasam V, Chandramohan N, Ragupathi N. Cultural and genetic variability in Macrophomina phaseolina (Tassi.) Goid. and incidence of mulberry root rot. Archives of Phytopathology and Plant Protection. 2010;43(2):123-132.
Bhupathi P, Theradimani, M. Studied on pathogen variability in black gram root rot by Macrophomina phaseolina. Pharma Inno. 2018;7:3638.
Kharde RR, Lavale SA, Ghorpade BB. Molecular diversity among the isolates of Xanthomonas axonopodis pv. citri causing bacterial canker in citrus. Int.J. Curr. Microbiol. Appl. Sci. 2018;7:2375-2384.
Nagendran K, Mohankumar S, Mohammed Faisal P, Bagewadi B, Karthikeyan, G. Molecular evidence for the occurrence of tomato leaf curl New Delhi virus on chayote (Sechium edule) in southern India. Virus disease. 2017;28:425-429.
Valerio HM, Resende MA, Weikert-Oliveira RCB, Casela CR. Virulence and molecular diversity in Colletotrichum graminicola from Brazil. Mycopathologia. 2005;159: 449-459.
Sharma PN, Padder BA, Sharma OP, Pathania A, Sharma P. Pathological and molecular diversity in Colletotrichum lindemuthianum (bean anthracnose) across Himachal Pradesh, a north-western Himalayan state of India. Australasian Plant Pathology. 2007;36(2):191-197.
Ndegwa E, O’Brien D, Matthew K, Wang Z, Kim J. Shiga toxin subtypes, serogroups, phylogroups, RAPD genotypic diversity, and select virulence markers of shiga-toxigenic escherichia coli strains from goats in mid-atlantic US. Microorganisms. 2022;10(9):1842.
Christopher DJ, Raj TS, Kumar R. Morphological, pathological and molecular variability of Colletotrichum capsici causing anthracnose of chilli. Annals of Plant Protection Sciences, 2013;21(2): 309-314.
Fry WE, Birch PRJ, Judelson HS, Grünwald NJ, Danies G, Everts KL, Smart CD. Five reasons to consider Phytophthora infestans a reemerging pathogen. Phytopathology. 2015;105(7):966-981.
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