Evaluation of Genetic Diversity in Indian Mustard (Brassica juncea var. rugosa) Employing SSR Molecular Markers
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 24, Issue 3-4,
Indian mustard (Brassica juncea var. rugosa) constitutes an authoritative group of mustard crops in India. Evaluation of genetic diversity is a vivacious component of mustard breeding programmes for efficient utilization of plant genetic resources. In present study, 77 microsatellite markers were employed to assess the genetic diversity of 75 Indian mustard genotypes. Results revealed positive amplification for all SSRs, with 21 SSRs exhibiting polymorphic amplicons. A total of 99 alleles, ranging from 3 to 5 with an average of 4.71 alleles per SSR marker were obtained. The major allele frequency varied between 0.26 (cnu/m616) and 0.56 (ENA28F) with an average value of 0.36. The average polymorphic information content (PIC) value was 0.67 and ranged between 0.43 (ENA28F) and 0.76 (gi258660710gbGT071338.1). Mean value of 0.72 was detected for each pair of SSR primers, with the gene diversity per locus ranging between 0.53 (ENA28F) and 0.79 (gi258660710gbGT071338.1). The dendrogram grouped the 75 genotypes into three main clusters or subpopulations based on Unweighted Neighbour-Joining technique. The study revealed better understanding of the genetic diversity among different Indian mustard genotypes using genomic-SSR markers that could be exploited for the genetic improvement of the crop.
- Brassica juncea
- simple sequence repeats
- genetic diversity
- polymorphic information content
How to Cite
Tandayu E, Borpatragohain P, Mauleon R, Kretzschmar T. Genome-wide association reveals trait loci for seed glucosinolate accumulation in Indian mustard (Brassica juncea L.). Plants. 2022; 11(3):364.
Shrivastava A, Tripathi MK, Solanki RS, Tiwari S, Tripathi N, Singh J, Yadav R. Genetic correlation and path coefficient analysis of yield attributing parameters in Indian mustard. Current Journal of Applied Science and Technology. 2023a; 42(7):42-58.
Kumar M, Choi JY, Kumari N, Pareek A, Kim S R. Molecular breeding in Brassica for salt tolerance: importance of microsatellite (SSR) markers for molecular breeding in Brassica. Frontiers Plant Science. 2015;6:688.
Redden R, Vardy M, Edwards D, Raman H, Batley, J. Genetic and morphological diversity in the Brassicas and wild relatives. 16th Australian Research Assembly on Brassicas. Ballarat, Victoria; 2009.
Shrivastava A, Tripathi MK, Singh P, Tiwari S, Tripathi N, Tiwari PN, Parihar P, Singh J, Chauhan S. Disease indexing of Indian mustard genotypes against Alternaria blight disease. The Pharma Innovation Journal. 2023b;12(4):08- 13.
Choudhary RL, Langadi AK, Jat, RS. Mitigating moisture stress in Indian mustard (B. juncea) through polymer. Journal of Oilseed Brassica. 2021; 12(1):21-27.
Meena PD, Awasthi RP, Chattopadhyay C, Kolte SJ, Kumar A. Alternaria blight: a chronic disease in rapeseed-mustard.Journal of Oilseed Brassica. 2016;1(1):1-11.
Barfa D, Tripathi MK, Kandalkar VS, Gupta JC, Kumar G. Heterosis and combining ability analysis for seed yield in Indian mustard [Brassica Juncea (L) Czern & Coss]. Ecology, Environment and Conservation. 2017;23 (February Suppl.):S75-S83.
Shyam C, Tripathi M K. Biochemical studies in Indian mustard [Brassica juncea (Linn) Czern & Coss] for fatty acid profiling. International Journal of Chemical Studies 2019; 7(4): 338-343.
Baghel R, Sharma A K, Tiwari S, Tripathi M K, Tripathi N. Genetic diversity analysis of Indian mustard (Brassica spp.) genotypes lines using SSR molecular markers. International Journal of Current Microbiology and Applied Science. 2020;9(12):137-143.
Singh SP, Pal MS. Effect of integrated nutrient management on productivity, quality, nutrient uptake and economics of mustard (Brassica juncea). Indian Journal of Agronomy. 2011;56(4): 381-387.
Shyam C, Tripathi MK, Tiwari S, Ahuja A, Tripathi N, Gupta N. In vitro regeneration from callus and cell suspension cultures in Indian mustard [Brassica juncea (Linn.) Czern & Coss].International Journal of Agricultural Technology. 2021a;17(3): 1095-1112.
Shyam C, Tripathi MK, Tiwari S, Tripathi N, Solanki RS, Sapre S, Ahuja A, Tiwari S. In vitro production of somaclones with decreased erucic acid content in Indian mustard [Brassica juncea (Linn.) Czern & Coss. Plants. 2021b;10:1297.
Shyam C, Tripathi MK, Tiwari S, Ahuja A, Tripathi N, Gupta N. Plant regeneration in Indian mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental investigation.In book: Current Topics in Agricultural Sciences. 2021c;3:120- 135. DOI: 10.9734/bpi/ctas/v3/2118C
Shyam C,Tripathi MK, Tripathi N, Tiwari S, Sikarwar RS. Genetic variations in fatty acids and oil compositions among 188 Indian mustard Brassica juncea (Linn.) Czern & Coss genotypes. Current Journal of Applied Science and Technology. 2022a;40(46):9-28 DOI: 10.9734/CJAST/2021/v40i4631629
Shyam C, Tripathi MK, Tripathi N, Tiwari S, Sikarwar RS. Analysis of genetic differences in fatty acids and oil contents among Brassica juncea (Linn.) Czern & Coss genotypes. In book: Research Developments in Science and Technology. 2022b;1:127-149. DOI: 10.9734/bpi/rdst/v1/6010F
Shyam C, Tripathi MK, Tiwari S, Tripathi N. Genetic components, and diversity analysis in Indian mustard [Brassica juncea (Linn.) Czern & Coss] based on different morpho-physiological traits. Current Journal of Applied Science and Technology. 2021d;40(20):34-57. DOI: 10.9734/CJAST/2021/v40i2031462
Shyam C, Tripathi MK, Tiwari S, Tripathi, N, and Sikarwar R S. Morpho-physiological variations and genetic components analysis in Brassica juncea (Linn.) Czern & Coss.In book: Research Developments in Science and Technology, 2022c;1:98-126. DOI: 10.9734/bpi/rdst/v1/6009F.
Shrivastava A, Tripathi MK, Tiwari S, Singh P, Tripathi N, Tiwari PN, Parihar P, Singh J, Chauhan S. Screening of Indian mustard genotypes against white rust disease based on disease indexing. Biological Forum – An International Journal. 2023c;15(4):268-272.
Shyam C, Tripathi MK, Tripathi N, Tiwari S, Sikarwar RS. Identification of low and high erucic acid containing genotype(S) in Indian mustard employing molecular markers.In book: Recent Progress in Plant and Soil Research. 2022d;5:18- 36. DOI:10.9734/bpi/rppsr/v5/15384D
Thakur AK, Singh KH, Singh L, Verma V, Singh D. Morphological and molecular descriptors for first Indian mustard hybrid. NRCHB. 2016;506.
Thakur AK, Parmar N, Singh KH, Nanjundan J. Current achievements and prospects of genetic engineering in Indian mustard (Brassica juncea L. Czern & Coss.). Planta. 2020;252:56.
Sharma D, Nanjundan J, Singh L, Singh SP, Parmar N, Sujith Kumar MS, Singh KH, Mishra AK, Singh R, Verma KS, Thakur AK. Genetic diversity in leafy mustard (Brassica juncea var. rugosa) as revealed by agro-morphological traits and SSR markers. Physiology and Molecular Biology of Plants. 2020;26:2005-2018.
Shyam C, Tripathi MK, Tiwari S, Tripathi N, Ahuja A. Molecular characterization and identification of Brassica genotype(s) for low and high erucic acid content using SSR markers. Global Journal of Bioscience and Biotechnology. 2020;9(2):56-66.
Chauhan JS, Singh KH, Singh VV, Kumar S. Hundred years of rapeseed-mustard breeding in India: accomplishments and future strategies. Indian Journal of Agriculture Sciences, 2011; 81: (12):1093–1109.
Tripathi MK, Tomar S S, Tiwari V K, Awasthi D, Gupta JC. Heterosis in Indian mustard [Brassica juncea (L) Czern and Coss]. Progressive Research. 2015;10, 3376-3379.
Thakur AK, Singh KH, Parmar N, Sharma D, Mishra DC, Singh L, Nanjundan J, Yadav, S. Population structure and genetic diversity as revealed by SSR markers in Ethiopian mustard (Brassica carinata A. Braun): a potential edible and industrially important oilseed crop. Genetic Resources and Crop Evolution. 2021;68:321-333.
Sharma D, Nanjundan J, Singh L, Parmar N, Singh KH, Verma KS, Thakur AK. Genetic diversity and population structure analysis in Indian Mustard germplasm using phenotypic traits and SSR markers. Plant Molecular Biology Reports. 2022;40:579–594.
Yadava DK, Yadav R, Vishwakarma H, Yashpal, Yadav S, Saini N, Vasudev S. Genetic diversity characterization and population structure in Brassica juncea. In: Kole C., Mohapatra T. (eds) The Brassica junceaGenome. Compendium of Plant Genomes. 2022;73-84. DOI:https://doi.org/10.1007/978-3-030-91507-0_5
Rajpoot NS, Tripathi MK, Tiwari S, Tomar RS, Kandalkar VS. Characterization of Indian mustard germplasm on the basis of morphological traits and SSR markers. Current Journal of Applied Science and Technology. 2020;39:300-311.
Verma K, Tripathi MK, Tiwari S, Tripathi N. Analysis of genetic diversity among Brassica juncea genotypes using morpho-physiological and SSR markers. International Journal of Current Microbiology and Applied Sciences. 2021;10:1108-1117.
Rajpoot NS, Tripathi MK, Tiwari S, Tomar RS, Tripathi N, Sikarwar RS, Tomar SS.. Morphological and molecular characterization of Indian mustard germplasm lines. Research and Development in Science and Technology. 2022;4:151-165.
Chand S, Singh N, Prasad L, Nanjundan J, Meena VK, Chaudhary R, Patel MK, Taak Y, Saini N, Vasudev S. Inheritance and Allelic Relationship among Gene(s) for White Rust Resistance in Indian Mustard [Brassica juncea (L.) Czern & Coss]. Sustainability. 2022;14:11620.
Mishra N, Tripathi MK, Tiwari S, Tripathi N, Gupta N, Sharma A, Solanki RS and Tiwari S. Characterization of soybean genotypes on the basis of yield attributing traits and SSR molecular markers. In book Innovations in Science and Technology. 2022a;3:87-106. DOI: 10.9734/bpi/ist/v3/2471C
Upadhyay S, Singh AK, Tripathi MK, Tiwari S, Tripathi N. Biotechnological interventions to combat against charcoal rot and Rhizoctonia root rot diseases of soybean [Glycine max (L.) Merrill].In book: Current Topics in Agricultural Sciences. 2022;6:1-18. DOI: https://doi.org/10.9734/bpi/ctas/v6/3250E
Rajpoot P, Tripathi MK, Tiwari S, Bimal SS, Tripathi N, Parihar P, Pandya RK, Tara Satyavathi C. Characterization of pearl millet [Pennisetum glaucum (l.) R br.] Genotypes against blast disease employing disease scoring and gene specific SSR markers. Scientists. 2023; 3(3):16-30.
Asati R, Tripathi M K, Tiwari S, Yadav RK, Tripathi N. Molecular breeding and drought tolerance in chickpea. Life. 2022; 12(11):1846.
Mishra N, Tripathi MK, Tripathi N, Tiwari S, Gupta N, Sharma A. Screening of soybean genotypes against drought on the basis of gene-linked microsatellite markers. In book Innovations in Science and Technology. 2022b;3:49-61. DOI: 10.9734/bpi/ist/v3/2454C.
Tripathi N, Tripathi MK, Tiwari S, Payasi DK. Molecular breeding to overcome biotic stresses in soybean: update. Plants (Basel). 2022;11(15):1967.
Yadav PK, Singh AK, Tripathi MK, Tiwari S, Yadav SK, Tripathi N. Morpho-physiological and molecular characterization of maize (Zea mays L.) genotypes for drought tolerance. European Journal of Applied Sciences. 2022;10(6):65-87.
Tripathi MK, Tripathi N, Tiwari S, Mishra N, Sharma A, Tiwari S, Singh S. Identification of Indian soybean (Glycine max [L.] Merr.) genotypes for drought tolerance and genetic diversity analysis using SSR markers. Scientists. 2023;3(3):31-46.
Yadav S, Tiwari S, Tripathi MK, Tripathi N, Gupta N, Tiwari S. Evaluation of high oleic acid content in a set of 96 genotypes of Arachis hypogaea L. Scientist. 2023a;2:132-143.
Yadav RK, Tripathi MK, Tiwari S, Tripathi N, Asati R, Patel V, Sikarwar RS, Payasi DK.Breeding, and genomic approaches towards development of Fusarium wilt resistance in chickpea. Life. 2023b;13: 988.
Zheng X, Cheng T, Yang L, et al. Genetic diversity and DNA fingerprints of three important aquatic vegetables by EST-SSR markers. Scientific Reports. 2019;9: 14074. DOI: https://doi.org/10.1038/s41598-019-50569-3
Devi YS, Devi TR, Thakur AK, Ngangkham U, Devi HN, Kh P, Sinha B, Senjam P, Singh NB, Mishra K. Evaluation of Indian mustard genotypes for white rust resistance using BjuWRR1 gene and their phenotypic performance. Agronomy. 2022;12:3122. DOI:https://doi.org/10.3390/ agronomy12123122
Paritosh K, Yadava SK, Singh P, Bhayana L, Mukhopadhyay A, Gupta V, Bisht NC, Zhang J, Kundma DA, Copetti D, Wing RA,Vijaya Reddy VBL, Pradhan AK, Penta D. A chromosome‐scale assembly of allotetraploid Brassica juncea (AABB) elucidates comparative architecture of the A and B genomes. Plant Biotechnology Journal. 2021;19(3): 602-614.
Murray MG, Thompson W. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980;8(19):4321-4326.
Tiwari S, Tomar RS, Tripathi MK, Ahuja, A. Modified protocol for plant genomic DNA isolation. Indian Research Journal of Genetics and Biotechnology. 2017; 9(4):478-485.
Liu K, Muse SV. Power Marker: new genetic data analysis software. Version 3.0; 2005. Avaiilable:http://www.powermarker.net
Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution. 2007;24(8):1596-1599.
Yao QL, Chen FB, Fang P, Zhou GF, Fan Y H, Zhang, Z R. Genetic diversity of Chinese vegetable mustard (Brassica juncea Coss) landraces based on SSR data. Biochemical Systematics and Ecology. 2012;45:41-48.
Fang P, Chen FB, Yao QL, Yan KC, Zhou GF, Fan YH, Shen JJ, Zang JR, Shen ZJ, Zang H. Analysis of genetic diversity in the tuber mustard (Brassica juncea var. tumida Tsen et Lee) in the Yangtze River basin of China. Genetic Resources and Crop Evolution. 2013;60:129-143.
Ghosh S, Mazumder M, Mondal B, Mukherjee A, De A, Bose R, Das S, Bhattacharyya S, Basu, D. Morphological and SSR marker-based genetic diversity analysis of Indian mustard (Brassica juncea L.) differing in Alternaria brassicicola tolerance. Euphytica. 2019; 215:1-19.
Abstract View: 0 times
PDF Download: 0 times