Effect of BAP (6-Benzylaminopurine) and NAA (α-Napthalene Acetic Acid) treatment on Micropropagation of Adansonia digitata L.
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 24, Issue 3-4,
Page 42-51
DOI:
10.56557/pcbmb/2023/v24i3-48292
Abstract
The African Baobab, also known as Kalpvriksha (Adansonia digitata L.), is a significant multi-purpose tree that has gained popularity among people, primarily in African countries, for its use as food, fodder, clothing, and ropes. There is one of the obstacle arises in growing this plant by farmers in India is the lack of planting materials. The young plants of A. digitata L. have to be imported form selected nurseries. Therefore, micropropagation can be used as a substitute method to create offspring that are genetically similar to the parent plant. In order to micropropagate Kalpvriksh (A. digitata L.), this experiment is being conducted utilizing shoot tip explants. For shoot proliferation, these explants are grown in MS medium containing various concentrations of BAP (6- Benzylamino purine): 0.5, 1.0, 1.5, 2.5, 5.0 mg/L. Shoots are rooted in a medium containing at the same concentration of NAA (α-Napthalene Acetic Acid) 0.5 mg/L alone or with combination. Complete Randomized Design (CRD) is used to conduct each experiment. ANOVA and the Duncan New Multiple Range test are used to examine the data. According to the technique for micropropagation of A. digitata L. The concentration of BAP and NAA was found to be 1.5 mg/L and 0.5 mg/L respectively which showed better enhancement for shoot regeneration and rooting for micropropagation of Kalpvriksha (Adansonia digitata L.) tree.
- Adansonia digitata L
- BAP & NAA
- Plant Growth Regulator (PGR)
- micropropagation
- shoot multiplication
How to Cite
References
Sidibe´ M, Williams JT. Baobab. Adansonia digitata L. Southampton: International Centre for Underutilised Crops; 2002.
Gebauer KE, Ebert G. Baobab (Adasonia digitata): A review on multipurpose tree with promising future in the Sudan); 2002. Available:http://www.gartenbauwisenchaft.org Retrieved 12/08/2010.
Rabi’u T, Garba KA, Ibrahim A, Murtala R. Indigenous trees inventory and their multipurpose uses in Dutsin-ma area Katsina State. European Scientific Journal. 2013;9(11):288-300.
Rabi’u T, Murtala R. An assessment of Adasonia digitata for sustainable development in the semi-urban regions of Dutsin-ma Katsina State Nigeria. Academic Research International. 2013; 4(1):2223-9944.
Lockett CT, Calvert CC, Grivetti LE. Energy and micronutrient composition of dietary and medicinal wild plants consumed during drought. Study of rural Fulani, Northeastern Nigeria. Int J Food Sci Nutr. 2000;51:195–208.
Besco E, Braccioli E, Vertuani S, Ziosi P, Brazzo F, Bruni R, et al. The use of photochemiluminescence for the measurement of the integral antioxidant capacity of baobab products. Food Chem. 2007;102:1352–1356.
Rashford J. Africa’s Baobab tree: Why monkey names? J. Ethnob. 1994;14:173–183.
Abdalla AA, Mohammed MA, Mudawi HA. Producton and quality assessment of instant Baobab (Adansonia digitata L.) Advanced Journal of Food Science and Technology. 2010;2(2):125-133.
Cuni Sanchez A, De Smedt S, Haq N, Samson R. Variation in baobab seedling morphology and its implications for selecting superior planting material. Sci Hortic. 2011;130:109–117.
United Nations. Market Brief in the European Union for Selected natural ingredients derived from native species Adansonia digitata L. (Baobab); 2005. Available: [http://www.unctad.org/biotrade/docs/biotradebri efbaobab.pdf].
Guèye EE. Ethnoveterinary medicine against poultry diseases in African villages. World’s Poultry Sci. J. 1999;55:187-198.
Assogbadjo AE, Kyndt T, Chadare FJ, Sinsin B, Gheysen G, Eyog- Matig O. Genetic fingerprinting using AFLP cannot distinguish traditionally classified baobab morphotypes. Agrof Syst. 2009;15:157–165.
Munthali CRY, Chirwa PW, Akinnifesi FK. Genetic variation among and within provenances of Adansonia digitata L. (Baobab) in seed germination and seedling growth from selected natural populations in Malawi. Agrof Syst. 2012;86:419–431.
Assogbadjo AE, Gle`le` Kakaı¨ R, Edon S, Kyndt T, Sinsin B. Natural variation in fruit characteristics, seed germination and seedling growth of Adansonia digitata L. in Benin. New Forests. 2011;41:113–125.
Cuni Sanchez A, Haq N, Assogbadjo AE. Variation in baobab (Adansonia digitata L.) leaf morphology and its relation to drought tolerance. Genet Resour Crop Evol. 2010;57:17–25.
Engelmann F, Engels JMM. Chapter 9 Technologies and Strategies for ex situ conservation. In: Engels JMM, Ramanatha Rao V, Brown ADH, Jackson MT, editors. Managing Plant Genetic Diversity. Oxford: CABI Publishing. 2002;89–104.
Freeman KLM, Bollen A, Solofoniaina FJF, Andriamiarinoro H, Porton I, Birkinshaw CR. The Madagascar Fauna and Flora Group as an example of how a consortium is enabling diverse zoological and botanical gardens tocontribute to biodiversity conservation in Madagascar. Plant Biosyst. 2014;148:570–580.
Rossi G, Orsenigo S, Dhital D, Shrestha S, Shrestha BB, Maharjan SR. Ex situ plant conservation initiative in developing country: Nepal as a case study. Plant Biosyst. 2014;148: 565–569.
Volis S, Blecher M. Quasi in situ: A bridge between ex situ and in situ conservation of plants. Biodivers Conserv. 2010;19:2441 2454.
Jensen JS, Bayala J, Sanou H, Korbo A, Ræbild A, Kambou S, et al. A research approach supporting domestication of Baobab (Adansonia digitata L.) inWest Africa. New Forests. 2011;41:317–335.
Ishii K, Kambou S. In vitro culture of an African multipurpose species Adansonia digitata L. Propag Ornam Plants. 2007;7:62–67.
Singh S, Rai S, Khan S. In vitro seed germination of Adansonia digitata L.: An endangered medicinal tree. Nanobiotech Univer. 2010;1:107–112.
Rolli E, Bigliardi MV, Ricci A. Micropropagation of Adansonia digitata L. Acta Italus Hortus. 2012;6:68–72.
N’Doye AL, Sambe MAN, Sy MO. Propagation of African Baobab (Adansonia Digitata L., Bombacoideae, Malvaceae) Germplasm through in vitro cloning. Adv Env Biol. 2012;6: 2749–2757.
Chen WC, Chang WP, Gow JT. Influence of growth regulators on direct embryo formation from leaf explants of Phalaenopsis orchids. Acta Physiol Plant. 2008;30:507-512.
Hermann EB. (1991-2006) Recent Advances in Plant Tissue Culture.
Hartmann HT, Kester T, Davies T. Plant Propagation, 7th edn. Prentice Hall, Englewood Cliffs, NJ; 2007.
Dixon RA, Gonzales RA. Plant Cell Culture: A Practical Approach. Oxford University Press, Oxford. UK. 1995;37-42.
Koning RE. Cytokinins. Plant Physiology Information Website; 1994.
Available:http://plantphys.info/plant_physiology/cytokinin.shtml
Uranbey S. Comparison of Kinetin and 6- benzyladenine (BA) on in vitro Micro tuberization of Potato Under Short Days Conditions. Centenary University, Faculty of Agriculture, Journal of Agricultural Sciences (J. Agric. Sci.). 2004;15(1):39- 41.
George EF. Plant Propagation by tissue culture. Handbook and Directory of commercial laboratories. Exegeties L.td. Eversely, Basingstoke, England; 2000.
Ameena Abdullah H, Al Maliki S, Suliman KM. Effect of medium strength and charcoal combined with IBA and NAA on root initiation of Ficus Anastasia; 2009.
Razdan MK. Introduction to Plant Tissue Culture.2nd edition Science Publishers,Inc. Enfield (NH),USA; 2005.
Hall RD. (ed.). Plant Cell Culture Protocols. Humana Press, Ottowa; 1999.
Filipecki M, Wisniewska A, Yin Z, Malepszy S. The heritable changes in metabolic profiles of plants regenerated in different types of in vitro culture. Plant Cell Tissue Organ Cult. 2005;82:349– 356.
Jaafar HZ, Black CR, Atherton JG, Roberts JA. Impact of water stress on reproductive development in sweet pepper (Capsicum annum L.) IV. Inhibition of CEPA induced ethylene; 1999.
Murashige T, Skoog F. A revised medium for rapid growth & bioassays with Tobacco Tissue Culture. Physiol. Plant. 1962;15:473-497.
Van Staden J, Zazimalova E, George EF. Plant growth regulators II: cytokinins, their analogues and antagonists. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht. 2008;205–226.
Sridhar TM, Aswath CR. Review onmedicinal plants propagation: a comprehensive study on role of natural organic extracts in tissue culture medium. Am J Plant Sci. 2014;5:3073–3088.
Biswas A, Bari MA, Roy M, Bhadra SK. Clonal propagation through nodal explant culture of Boerhaavia diffusa L.-a rare medicinal plant. Plant Tissue Cult Biotechnol. 2019;19:53–59.
Bonga JM, Aderkas PV. In vitro culture of Trees; Collection, Sterilization, Excision and Culture. Publisher: Kluwer Academic Publishers. 1992;38:58-59.
Jain SM, Jenks MA, Rout GR, Radejovic L. Micropropagation of ornamental potted plants. Prop. Orn. Plants. 2006;6:67-82.
Stephen I. The man who knew too much: the strange and inventive life of Robert Hooke, 1635 1703. London: Pan. 2003;72.
Azizan MN, Zakaria NL. The effect of BAP and NAA treatment on micropropagation of Cucumis sativus L. International Journal of Science and Research (IJSR). 2017;6(11).
-
Abstract View: 0 times
PDF Download: 0 times
